期刊
VIRAL IMMUNOLOGY
卷 36, 期 2, 页码 136-143出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/vim.2022.0149
关键词
FOXP3; rs2232365; HTLV-1; HAM
This study evaluated the association between the polymorphism rs2232365 in the promoter region of the FOXP3 gene and HTLV-1-associated myelopathy (HAM). The results showed that individuals with the GG genotype of rs2232365 had higher proviral loads and CD4(+) T lymphocyte concentrations.
Human T lymphotropic virus 1 (HTLV-1) is a retrovirus associated with inflammatory diseases, including HTLV-1-associated myelopathy (HAM), and host genetic factors may be involved in disease evolution. The forkhead Box P3 (FOXP3) transcription factor is linked to homeostasis of the immune system, and the presence of polymorphisms in the promoter region of the FOXP3 gene should reflect its expression levels and consequent activation of regulatory T cells, which may contribute to severe inflammatory disorders, such as HAM. This study evaluated the rs2232365 polymorphism (-924 A/G) located in the promoter region of the FOXP3 gene and its association with HAM. Forty DNA samples from asymptomatic carriers and 25 samples from HAM patients were used, in addition to 130 control samples. The polymorphism was genotyped by conducting real-time polymerase chain reaction (PCR) (quantitative PCR [qPCR]) on extracted DNA. The proviral loads (PVLs) and CD4(+) and CD8(+) T lymphocyte counts were determined by qPCR and FACSCalibur flow cytometry, respectively. The PVLs, CD4(+) T lymphocyte concentrations, and tumor necrosis factor-alpha dosages were considered predictive factors of the clinical profiles of HTLV-1 infection, all of which had higher levels in the HAM group. Carriers of the GG genotype for the polymorphism rs2232365 had high PVLs and CD4(+) T lymphocyte concentrations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据