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Comparison of CRISPR/Cas and Argonaute for nucleic acid tests

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TRENDS IN BIOTECHNOLOGY
卷 41, 期 5, 页码 595-599

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CELL PRESS
DOI: 10.1016/j.tibtech.2022.11.002

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This study aims to compare the similarities, differences, and complementarities between the CRISPR/Cas system and Argonaute (Ago) as nucleases, in order to guide the design of novel nucleic acid tests.
Guided, programmable, and target-activated nucleases, exemplified by Cas in the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system and Argonaute (Ago), are emerging as a new generation of nucleic acid tests (NATs). A specific approach for comparison of these two nucleases side by side in terms of similarities, differences, and complementarities is instru-mental for the sensible design of novel NATs.

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