Aptamer-based biosensors for virus protein detection

Virus threatens life health seriously. The accurate early diagnosis of the virus is vital for clinical control and treatment of virus infection. Aptamers are small single-stranded oligonucleotides (DNAs or RNAs). In this review, we summarized aptasensors for virus detection in recent years according to the classification of the viral target protein, and illustrated common detection mechanisms in the aptasensors (colorim-etry, fluorescence assay, surface plasmon resonance (SPR), surface-enhanced raman spectroscopy (SERS), electrochemical detection, and field-effect transistor (FET)). Furthermore, aptamers against different target proteins of viruses were summarized. The relationships between the different biomarkers of the viruses and the detection methods, and their performances were revealed. In addition, the challenges and future directions of aptasensors were discussed. This review will provide valuable references for constructing on-site aptasensors for detecting viruses, especially the SARS-CoV-2.(c) 2022 Elsevier B.V. All rights reserved.

Automated summary

Viruses pose a serious threat to human life and health. Accurate early diagnosis is crucial for controlling and treating virus infections. In this review, the application of aptasensors for virus detection in recent years is summarized. The common detection mechanisms and the use of aptamers are illustrated. The relationships between virus biomarkers, detection methods, and their performance are revealed. Furthermore, the challenges and future directions of aptasensors are discussed.