期刊
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 158, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2022.116836
关键词
Loop -mediated isothermal amplification; Centrifugal microfluidic chips; Chip construction; DNA extraction; Fluid control; Signal production; Portable device; Point-of-care
Loop-mediated isothermal amplification (LAMP) is a widely used thermostatic nucleic acid amplification technology in clinical diagnosis and food safety detection. Centrifugal microfluidic chips, developed based on traditional microfluidic chips, utilize centrifugal force for liquid flow, mixing, and reaction, eliminating the need for complex valve and pump design. The combination of LAMP and centrifugal microfluidic chips holds great potential for rapid on-site nucleic acid detection.
Loop-mediated isothermal amplification (LAMP) is a thermostatic nucleic acid amplification technology that is widely used in the fields of clinical diagnosis and food safety detection. Centrifugal microfluidic chips are developed based on traditional microfluidic chips, using centrifugal force to achieve liquid flowing, mixing and reaction, eliminating the design of complex valves and pumps. The combination of LAMP and centrifugal microfluidic chips has great potential for rapid field nucleic acid detection. In this review, we discuss the design of a centrifugal microfluidic chip based on LAMP technology, including the chip fabrication, DNA extraction chamber, fluid control technology, common dye types in the reaction chamber, and the supporting detection device. It aims to provide some ideas and references for the further development and application of centrifugal microfluidic chip-based loop-mediated isothermal amplification in fast, on-site nucleic acid detection such as point-of-care tests and food safety. & COPY; 2022 Elsevier B.V. All rights reserved.
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