Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics

The envenomation from the Bothrops genus is characterized by systemic and local effects caused by the main toxin families in the venom. In Bothrops pubescens venom we were able to identify 89 protein groups belonging to 13 toxin families with the bottom-up proteomics approach and 40 unique proteoforms belonging to 6 toxin families with the top-down proteomics approach. We also identified multi-proteoform complexes of dimeric L-amino acid oxidase using native top-down mass spectrometry.

Automated summary

Envenomation from the Bothrops genus is characterized by the effects of toxin families in the venom, which can be identified using proteomics approaches. Various protein groups, proteoforms, and multi-proteoform complexes have been identified in Bothrops pubescens venom.