期刊
TOXICOLOGY LETTERS
卷 372, 期 -, 页码 14-24出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2022.10.003
关键词
Lipid-nephrotoxicity; Necroptosis; Phosphoglycerate mutase 5 (PGAM5); Dynamin-related protein 1(DRP1); Mitophagy
类别
This study investigates the mechanism of PGAM5 in promoting lipid-induced necroptosis in renal tubular epithelial cells (RTECs). The study finds that upregulation of PGAM5 activates the necroptosis pathway, leading to cell death. However, silencing PGAM5 can prevent these changes, protecting RTECs from lipid-induced necroptosis.
The pathophysiology of renal lipid toxicity caused by excess adiposity is not well-understood. Necroptosis, a regulated form of cell death, is involved in injuring renal tubular epithelial cells (RTECs). Phosphoglycerate mutase 5 (PGAM5) is a key downstream effector of necroptosis. This study investigated the underlying mechanism of PGAM5 in promoting lipid-induced necroptosis in RTECs. HK2 cells (an immortalized proximal tubule epithelial cell line) were exposed to oleic acid (OA) to mimic the lipid overload environment in vitro. We found that OA suppressed HK2 cell proliferation, triggered cytoskeleton rupture and cell death. In OA-treated cells, upregulated expression of necroptosis pathway proteins, phosphorylated receptor-interacting protein-1/3 (pRIPK1/3), phosphorylated mixed lineage kinase domain-like protein (pMLKL), PGAM5, phosphorylated dynamin-related protein 1 (pDRP1S616), and downregulated pDRP1S637 expression were observed. This was accompanied by mitochondrial dysfunction (mitochondrial ROS overproduction and decreased mitochondrial membrane potential) and increased cellular necrosis, as reflected by Annexin V/ Propidium Iodide (PI) labeling. OA also induced the accumulation of LC3II and P62, blocking autophagosome fusion with lysosomes. Knockdown of PGAM5 could prevent these OA-induced changes. We propose inhibition of PGAM5 protects lipidinduced RTECs from necroptosis by reducing DRP1-mediated mitochondrial fission and improving mitophagy flux.
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