4.5 Article

A novel binding site in the nicotinic acetylcholine receptor for MB327 can explain its allosteric modulation relevant for organophosphorus-poisoning treatment

期刊

TOXICOLOGY LETTERS
卷 373, 期 -, 页码 160-171

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ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2022.11.018

关键词

nAChR; Cooperativity; Bispyridinium compounds; Blind docking; Molecular dynamics simulations; Rigidity analysis

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Organophosphorus compounds (OPCs) are highly toxic and can block acetylcholine esterase (AChE), leading to overstimulation of acetylcholine receptors (nAChRs). Current treatment options are insufficient, necessitating the development of new therapeutic approaches. The bispyridinium non-oxime MB327 has been found to interact directly with nAChRs, potentially through allosteric mechanisms. In this study, a new allosteric binding pocket (MB327-PAM-1) for MB327 was identified at the transition of the extracellular to the transmembrane region. The interaction of MB327 at this site suggests its potential as a novel treatment target, as it can interact with multiple nAChR subtypes from different species. Furthermore, modifications of MB327 resulted in more potent resensitizers PTM0062 and PTM0063.
Organophosphorus compounds (OPCs) are highly toxic compounds that can block acetylcholine esterase (AChE) and thereby indirectly lead to an overstimulation of muscarinic and nicotinic acetylcholine receptors (nAChRs). The current treatment with atropine and AChE reactivators (oximes) is insufficient to prevent toxic effects, such as respiratory paralysis, after poisonings with various OPCs. Thus, alternative treatment options are required to increase treatment efficacy. Novel therapeutics, such as the bispyridinium non-oxime MB327, have been found to reestablish neuromuscular transmission by interacting directly with nAChR, probably via allosteric mechanisms. To rationally design new, more potent drugs addressing nAChR, knowledge of the binding mode of MB327 is fundamental. However, the binding pocket of MB327 has remained elusive. Here, we identify a new potential allosteric binding pocket (MB327-PAM-1) of MB327 located at the transition of the extracellular to the transmembrane region using blind docking experiments and molecular dynamics simulations. MB327 forms striking interactions with the receptor at this site. The interacting amino acids are highly conserved among different subunits and different species. Correspondingly, MB327 can interact with several nAChR subtypes from different species. We predict by rigidity analysis that MB327 exerts an allosteric effect on the orthosteric binding pocket and the transmembrane domain after binding to MB327-PAM-1. Furthermore, free ligand diffusion MD simulations reveal that MB327 also has an affinity to the orthosteric binding pocket, which agrees with recently published results that related bispyridinium compounds show inhibitory effects via the orthosteric binding site. The newly identified binding site allowed us to predict structural modifications of MB327, resulting in the more potent resensitizers PTM0062 and PTM0063.

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