4.7 Article

Nucleus-targeting pH-Responsive carbon dots for fast nucleus pH detection

期刊

TALANTA
卷 252, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.talanta.2022.123855

关键词

Nucleus pH detection; Carbon quantum dots; Biosensor; Nucleus tracking; Long -rang pH sensor; In -vivo pH sensor

向作者/读者索取更多资源

This article reports a fluorescent sensor that can directly monitor changes in nucleus pH, which is of great significance in understanding nucleus-related biological events.
Nucleus pH is closely linked to many diseases such as aging, heart disease, skeletal myopathies, cancer, Alz-heimer's disease, etc. Nevertheless, fluorescent sensors that can directly monitor nucleus pH changes have not yet been reported. Here, we first reported a green emissive carbon dots (CDs) for nucleus pH detection in living cells. CDs can selectively target nucleus with high accumulation at nucleolus due to their high affinity towards RNA once entering cells by lipid raft mediated endocytosis. Without washing, CDs at 5 mu g/mL was enough to lighten nucleus within 10 min with the fluorescence on ever after 24 h incubation, achieving fast, wash-free, and long-term nucleus/nucleolus imaging. Meanwhile, the luminescent intensity of CDs was reduced gradually when pH changed continuously from 1 to 12, showing a pH-responsive fluorescence property with two linear ranges of pH 2-7 and pH 7-12. With their nucleus-targeting ability and pH-dependent photoluminescent property, CDs was successfully leveraged for nucleus pH detection in A549 cells and for in vivo pH sensing in zebra fish. CDs present a promising and powerful fluorescent sensor for nucleus imaging and nucleus pH sensing in living cells on the way to understand nucleus-related biological events.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据