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Article
Chemistry, Analytical
Wanwisa Deenin et al.
Summary: In this study, an integrated lateral-flow immunoassay with an electrochemical readout (eLFIA) was developed for early-stage detection of LipL32, a protein present only in pathogenic Leptospira species. The eLFIA showed superior performance in terms of a low detectable limit and a broad linear dynamic range. The test strip provided a straightforward and sensitive approach for the diagnosis of leptospirosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Jianyuan Zhao et al.
Summary: COVID-19 is a global pandemic caused by a novel coronavirus. This study identified 4 potential antiviral drugs, with 5-Iodotubercidin exhibiting the strongest inhibition of the virus's RNA-dependent RNA polymerase. Further investigation showed that the antiviral activity of 5-Iodotubercidin is closely related to its inhibition of adenosine kinase.
ANTIVIRAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Lluc Farrera-Soler et al.
Summary: The early phase of the SARS-CoV-2 pandemic posed a significant diagnostic challenge. In this study, a method combining recombinase polymerase amplification and a DNA-templated reaction was developed for dual readout detection of the virus, using either fluorescence or naked eye detection. This method offers a potential solution for rapid and accurate detection of SARS-CoV-2.
Article
Biophysics
Yong Sun et al.
Summary: This work introduces a new detection technology that can simultaneously test for SARS-CoV-2 and influenza viruses, with advantages such as speed, high sensitivity, no cross-reactivity, and false positives. Its clinical application demonstrates high accuracy, and this portable device is expected to meet the global healthcare system's need for online monitoring of serious infectious diseases.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Jianghua Jia et al.
Summary: The study presents a novel fluorescent LFIA method with improved sensitivity and specificity for reliable and highly-sensitive SARS-CoV-2 antibody diagnosis, which is crucial for extensive COVID-19 screening and control.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Changsheng He et al.
Summary: The increasing prevalence of SARS-CoV-2 variants with spike mutations has raised concerns. Rapid and accurate detection of these variants is crucial for infection prevention and control. The RT-CORDS platform accurately, sensitively, specifically, and rapidly detects SARS-CoV-2 variants, making it a potential tool for clinical diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Engineering, Environmental
Neeraj Kumar et al.
Summary: This review covers various methods for detecting COVID-19, including rapid antigen tests, RT-PCR, and electrochemical sensors, focusing on the development and application of electrochemical sensors for rapid monitoring and detection of SARS-CoV-2. It also compares and assesses their detection limits, sensitivities, and specificities for the identification and monitoring of COVID-19.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Yuanfeng Pang et al.
Summary: By combining CRISPR-Cas12a and SERS technology, the sensitivity and specificity of LFA nucleic acid detection have been improved, allowing for direct quantification of HIV-1 dsDNA in less than 1 hour and recognition of HIV-1 single-base drug resistance mutations. This paper-based CRISPR-SERS strip has great potential for point-of-care testing in resource-poor or non-laboratory environments.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Immunology
Makoto Kurano et al.
Summary: This study investigated the antibody responses to SARS-CoV-2 in Japanese individuals with COVID-19. The results showed differences in antibody responses between Japanese and Western populations. IgA antibodies were the first to appear after infection, and IgG and/or IgA seroconversion often preceded IgM seroconversion. The duration of IgG responses to viral proteins and responses of all three antibody classes to S1 and RBD were longer compared to IgA/IgM responses to viral proteins and responses of all three antibody classes to NCP.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Letter
Infectious Diseases
Xiquan Yan et al.
JOURNAL OF INFECTION
(2022)
Article
Chemistry, Analytical
Han Han et al.
Summary: A dual-functional lateral flow immunoassay biosensor was developed for rapid and sensitive detection of SARS-CoV-2, using colorimetric and fluorescent signals. The biosensor showed good repeatability, specificity, and accuracy, making it a potential tool for rapid detection of SARS-CoV-2.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Nan Zhang et al.
Summary: This paper reviews the biological and ecological toxicities caused by small molecular contaminants in food and environment, as well as the development of aptasensors in recent years. It also discusses in detail the signal enhancing strategies based on nanomaterials and nucleic acid amplification, along with the challenges and future trends in the application of these strategies.
Review
Medicine, Research & Experimental
Xirui Chen et al.
Summary: This article introduces the application of engineered noble metal nanoparticles in improving immunoassay sensitivity. By modulating the physical parameters of nanoparticles, including size, shape, composition, and external structure, the performance of immunoassay can be improved. Engineered nanoparticles have a wide range of applications in trace analysis.
Article
Biochemical Research Methods
Zahir Ali et al.
Summary: Bio-SCAN is a rapid and accurate pathogen detection platform for SARS-CoV-2, offering high sensitivity and efficiency in distinguishing between different variants.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Materials Science, Biomaterials
Liya Ye et al.
Summary: In order to quickly evaluate the immune efficacy of COVID-19 vaccines, researchers have developed a new detection method based on colloidal gold immunochromatographic assay. This method is capable of rapidly detecting SARS-CoV-2 total antibodies within 15 minutes and shows good correlation with the results from traditional ELISA method.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Chemistry, Multidisciplinary
Sang Hoon Kim et al.
Summary: GlycoGrip is a lateral flow assay developed based on the role of cell-surface glycoproteins as coreceptors for pathogens, allowing for the detection of SARS-CoV-2 and its variants. By utilizing glycopolymers and antibody-gold nanoparticle conjugates, it achieves sensitive and rapid virus detection. Through computational optimization, the test strips have been fine-tuned to selectively detect the virus in biofluids.
ACS CENTRAL SCIENCE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Julia Gaspar de Oliveira Santos et al.
Summary: This study aimed to evaluate the impact of SARS-CoV-2 on saliva composition, revealing decreased lactoferrin and IgA concentrations in COVID-19 patients, and increased IgM concentrations during acute infection.
JOURNAL OF ORAL BIOSCIENCES
(2022)
Article
Chemistry, Analytical
H. F. El Sharif et al.
Summary: This study investigates the use of electropolymerized molecularly imprinted polymers (E-MIPs) for the selective recognition of SARS-CoV-2 whole virus. The results show that E-MIPs exhibit selectivity for SARS-CoV-2, allowing for rapid detection and effective capture of the virus in real patient saliva samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Bryan Gosselin et al.
Summary: A dipstick assay using silver nanoparticles stabilized by a thin calix[4]arene-based coating was developed for detecting Anti-SARS-CoV-2 IgG in clinical samples. Compared to traditional lateral-flow immunoassays using gold nanoparticles, this silver nanoparticle-based system showed a lower limit of detection and similar signals with a smaller number of particles. The dipstick assay demonstrated impressive results in real clinical samples, with 100% specificity for negative samples and a sensitivity of 73% for positive samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Lucy F. Yang et al.
Summary: In this study, researchers identified a new DNA aptamer (SNAP4) that binds with high affinity to the SARS-CoV-2 spike protein. AptaFlow, a lateral flow assay using both SNAP1 and SNAP4, was able to detect UV-inactivated SARS-CoV-2 virus at concentrations as low as 106 copies/mL.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Anle Ge et al.
Summary: The study developed a device called PaGeR for rapid and simple detection of COVID-19. The device uses four simple steps to achieve rapid detection of SARS-CoV-2, and can detect the virus selectively with naked-eye. It supports multiple detection methods including colorimetric, fluorometric and lateral dipstick readout. Experimental results with clinical RNA samples showed that the instrument can effectively diagnose COVID-19 patients.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Qin Zhang et al.
Summary: The development of reliable and fast diagnostic devices for COVID-19 is crucial. In this study, a new analysis principle combining reverse transcription and isothermal amplification with CRISPR/Cas13a reaction was proposed. The method showed good sensitivity and specificity in detecting the SARS-CoV-2 gene fragment, and results could be obtained within 1 hour using a portable device. This approach has the potential for point-of-care screening in remote areas with limited resources.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Ketan Dighe et al.
Summary: The COVID-19 pandemic has highlighted the shortcomings in healthcare systems globally when it comes to rapid and accurate diagnosis. This study introduces a lateral flow strip-based RNA extraction and amplification-free nucleic acid test (NAT) for the rapid diagnosis of COVID-19 cases. The assay demonstrates high accuracy and specificity.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Xuejun Duan et al.
Summary: This article introduces a dual-detection fluorescent immunochromatographic assay (DFIA) for rapid quantitative detection of neutralizing antibodies against SARS-CoV-2. The assay uses a self-calibration process and demonstrates improved precision, sensitivity, and a wider dynamic linear range.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Hyun-Kyung Oh et al.
Summary: This study developed AuNP clusters to improve the detection sensitivity of lateral flow immunoassays (LFI) for diseases, including SARS-CoV-2. The AuNP clusters maintained the red color of isolated individual AuNPs while increasing the colorimetric readout. The PLASCOP AuNP clusters showed high sensitivity and good specificity for SARS-CoV-2 nucleocapsid proteins, making them a promising sensing tool for LFI applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Liang Qu et al.
Summary: This study reports a circular RNA vaccine that provides potent protection against SARS-CoV-2 by eliciting neutralizing antibodies and T cell responses. The circRNA vaccine exhibits higher and more durable antigen production compared to mRNA vaccines and shows efficacy against the current variants of concern.
Article
Biochemistry & Molecular Biology
Wajihul Hasan Khan et al.
Summary: This study demonstrates the significant impact of dimerization of the nucleocapsid protein on sensitivity of COVID-19 diagnostic assays. The dimeric form shows higher recognition by specific monoclonal antibodies and exhibits greater stability and antigenicity compared to the monomeric form. Manufacturers should maintain the monomer-dimer composition for accurate and robust diagnostics.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Icaro Putinhon Caruso et al.
Summary: This study investigates the binding characteristics of the SARS-CoV-2 nucleocapsid protein with nucleic acid sequences and reveals its role in RNA transcription and liquid-liquid phase separation. The findings suggest that the binding of the nucleocapsid protein to a specific sequence can induce DNA melting, which is crucial for viral infection. Additionally, the study shows a preference for the nucleocapsid protein to form liquid condensates with specific sequences, providing insights for the design of drugs targeting the nucleocapsid protein.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Mahamaya Biswal et al.
Summary: The interaction between the nucleocapsid (N) protein of SARS-CoV-2 and G3BP1 plays a crucial role in suppressing stress granule formation and promoting viral production. This study provides insights into the molecular basis of the interaction and its potential implications for developing therapeutic strategies against SARS-CoV-2 infection.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yuen Yung Hui et al.
Summary: In this study, a low-cost and portable lateral flow immunoassay (LFIA) reader based on photothermal detection was developed. The LFIA reader utilized a low-power green laser as the heating source and a single-element infrared sensor as the detector for accurate quantitative detection of reporters captured on LFIA strips. The results showed that photothermal detection significantly enhanced the sensitivity of LFIA and enabled quantitative analysis to support critical decision-making.
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
(2022)
Article
Biochemical Research Methods
Ye Xu et al.
Summary: A magnetic particle-based chemiluminescence enzyme immunoassay (CLEIA) was developed for the measurement of SARS-CoV-2-NP. The method showed high sensitivity and linearity, making it a potential diagnostic tool for clinical measurement.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Nanoscience & Nanotechnology
Xiaofei Jia et al.
Summary: We developed SERS-LFIA biosensor strips based on SiO2@Au nanoparticles for the specific and highly sensitive detection of ricin, SEB, and BoNT/A, achieving improved performance and rapid detection.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Analytical
Guihua Jiang et al.
Summary: In this study, a multifunctional carbon nanodots-decorated plasmonic Au@Ag nanoenzymes coupled with Ti2C/Ag2S composites were developed for photoelectrochemical biosensing of microRNA. The sensor demonstrated a wide linear range and low limit of detection, and the engineered nanoenzymes offered environmentally friendly and multifunctional signal amplification. This research has potential applications in biomarker detection and disease diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Immunology
M. A. Sughayer et al.
Summary: This study compares the efficacy and duration of anti-RBD IgG antibody response induced by different COVID-19 vaccines, showing a decrease in antibody titers after four months and recommending a booster shot with a more effective vaccine at that time.
Article
Cell Biology
Athena Labeau et al.
Summary: This study identifies 107 cellular factors that interact with the SARS-CoV-2 genome during infection and demonstrates that HNRNPA2B1, ILF3, QKI, and SFPQ promote viral RNA amplification. These findings provide valuable resources for future investigations into SARS-CoV-2 replication mechanisms and the development of host-centered antiviral therapies.
Article
Biology
Eltayib H. Ahmed-Abakur et al.
Summary: In this study, genetic variations among SARS-CoV-2 variants isolated in India were identified and their possible impacts on the virus's structural and functional characteristics were determined. The S gene showed the most frequent mutations, with the D614G mutation being the most common.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Medicine, General & Internal
Alessandra Ruggiero et al.
Summary: A longitudinal study was conducted on healthcare workers who received the BNT162b2 vaccine to quantify anti-SARS-CoV-2 IgG and IgM levels. It was found that coordinated expression of IgG-S and IgM-S after vaccination was associated with a more efficient response in both antibody levels and virus-neutralizing activity. The unconventional responses may suggest a recruitment of cross coronaviruses immunity by vaccination, warranting further investigation.
Article
Parasitology
Jyoti S. Kumar et al.
Summary: A rapid and sensitive RT-LAMP assay for detection of CCHFV has been developed in this study, which can be completed in a short time, providing crucial information for outbreak control and clinical management.
Article
Biophysics
Yongchun Pan et al.
Summary: This study develops a CRISPR/aptamer-based sensor, sheltered by a hollow COF, for ATP imaging in living animals. This strategy not only expands the applications of the CRISPR-Cas system, but also provides potential for the use of other proteins in clinical diagnostics, medical research, and biomimetic nanodevices.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Medical Laboratory Technology
Chang Chu et al.
Summary: Both infection with and vaccination against SARS-CoV-2 can trigger a complex B-cell and T-cell response. Reliable methods for analyzing the B-cell response are well established, but methods for measuring the T-cell response still need to be proven in clinical settings. In this study, a T-cell proliferation assay based on 3H thymidine incorporation was developed and validated. The assay uses SARS-CoV-2 derived peptide pools to stimulate T-cell responses. The novel SARS-CoV-2 lymphocyte transformation test (SARS-CoV-2 LTT) showed excellent discrimination of infected and/or vaccinated individuals versus unexposed controls, with an area under the curve (AUC) of >0.95 in ROC analysis. Furthermore, the assay can also differentiate T-cell responses resulting from vaccination against those induced by infection by using peptide pools representing not only the S protein but also the N and M proteins.
CLINICA CHIMICA ACTA
(2022)
Article
Computer Science, Artificial Intelligence
Jayanth Sivakumar et al.
Summary: This study proposes an automated framework for addressing the challenges of wearable lateral flow assay (LFA) devices on smartphones through image processing steps. The framework accurately identifies wearable sensors under various conditions and detects biomarker lines in the LFA. A strategy to handle reflection and different illumination conditions improves the sensitivity of the framework, which uses real-time images for analysis.
EXPERT SYSTEMS WITH APPLICATIONS
(2022)
Article
Infectious Diseases
Jinfeng Li et al.
Summary: A quantum dot lateral flow immunoassay strip (QD-LFIA) for smartphones was designed to detect specific IgG or neutralizing antibodies in SARS-CoV-2. The results showed high sensitivity and specificity in detecting antibodies in recovered patients and vaccinated individuals, with no cross-reaction observed.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Engineering, Environmental
Huiyi Yang et al.
Summary: Multimodal lateral flow immunoassay (LFIA) is a method that improves the practicality and flexibility of point-of-care testing. In this study, multifunctional core-shell-shell Au@Pt@Ag NPs loaded with dual-layer Raman reporter molecules were constructed for colorimetric LFIA (CM-LFIA), photothermal LFIA (PTLFIA), and surface-enhanced Raman scattering-based LFIA (SERS-LFIA), respectively. The nanoprobes showed excellent sensing sensitivity and were successfully used in multimodal LFIA with one visual qualitative and two optional quantitative modes. The developed LFIA demonstrated high detection sensitivity and good performance in real samples, indicating its great potential for accurate tracing of hazardous analytes in complex samples.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Analytical
Fanglan He et al.
Summary: This study developed a portable and multiple miRNAs detection method, combining rolling circle amplification with fluorescent microspheres based lateral flow assay. A smartphone-based strip reader with a WeChat mini program was used to analyze the fluorescence intensity of test lines and control lines on strips for point-of-care testing.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Zihao Xie et al.
Summary: A novel magnetic/fluorescent dual-modal lateral flow immunoassay has been developed for the rapid and accurate diagnosis of SARS-CoV-2 nucleocapsid protein. This method combines the advantages of magnetic and fluorescent signals, resulting in high sensitivity and accuracy, and shows potential for early-stage diagnosis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Gaoxing Su et al.
Summary: The orthogonal CRISPR-Cas-mediated multiplexed lateral flow assay (OC-MLFA) proposed in this study for SARS-CoV-2 genome detection takes advantage of activation and cleavage preferences between Cas12a and Cas13a, achieving high accuracy and efficiency. This method features simultaneous detection of multiple genes in one reaction and colorimetric readout through a single strip, showing promising feasibility for field-deployable point-of-care testing applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Gaihua Cao et al.
Summary: This study developed a biosensor based on CRISPR/Cas13a and RPA technology to detect the S and Orf1ab genes of SARS-CoV-2 quickly and accurately. Additionally, an automated, portable fluorescence analyzer and lateral flow strip were developed to provide more accurate and high-throughput detection results during on-site testing.
Article
Biochemistry & Molecular Biology
Kunchur Guruprasad
Summary: The study compared over 300,000 human SARS-CoV-2 spike protein sequences and found that 96.5% of the sequences have undergone mutations since the outbreak of the pandemic. A total of 1,229 distinct mutation sites were identified, but 3.5% of the sequences remained unchanged.
CURRENT RESEARCH IN STRUCTURAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Yunxiang Wang et al.
Summary: A low-cost and highly sensitive diagnostic platform is reported, which can simultaneously distinguish wild-type (WT) SARS-CoV-2 and its two mutations within 2 hours. The platform utilizes PCR amplification and a dual-color fluorescent test strip to detect the specific nucleic acid fragments, with results interpreted by the naked eye or a smartphone-connected device. This method provides a simple, accurate, and affordable approach for screening SARS-CoV-2 and its variants, even in resource-limited settings.
Article
Biophysics
Yaofeng Zhou et al.
Summary: The PEI-CISG technology introduced in this study controls the growth of copper nanoshells on the surface of gold nanoparticles in AuNP-LFS, effectively enhancing the detection sensitivity for various infectious diseases.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Virology
M. Shaminur Rahman et al.
Summary: The study characterized mutations in the nucleocapsid (N) protein of SARS-CoV-2, identifying a significant number of nucleotide and amino acid mutations. Particularly noteworthy were mutations at primer binding sites and high-frequency mutations impacting the protein's structure.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Review
Chemistry, Analytical
Yibo Yan et al.
Summary: This article summarizes the application potential of aggregation-induced emission (AIE) in forensic science and its outstanding performance in on-site analysis, chemical analysis, and bio-sensing. AIE can be used not only for detecting toxic substances and hazardous materials, but also play a role in forensic genetics, forensic pathology, and criminal investigations.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Erhu Xiong et al.
Summary: The newly developed method can simultaneously detect two SARS-CoV-2 genes in a single test, showing high sensitivity and accuracy, providing a more accurate and convenient pathway for COVID-19 diagnosis in low-resource regions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biophysics
Aldo Roda et al.
Summary: The study developed a noninvasive and highly sensitive immunosensor for IgA in saliva and serum, which can be used for diagnosing and monitoring COVID-19 infection. The immunosensor demonstrated the capability to detect salivary IgA in infected individuals and correlated with disease severity and infection time.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Jong-Hwan Lee et al.
Summary: This study presents a novel rapid detection method for the SARS-CoV-2 S1 antigen using a matched pair of ACE2 and antibody. The detection method showed high specificity and sensitivity without cross-reactivity, making it a valuable tool in detecting the S1 antigen from COVID-19 patients.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biotechnology & Applied Microbiology
Gizem Tatar et al.
Summary: This study investigated the potential of repurposing antiviral compounds against SARS-CoV-2 nucleocapsid protein, identifying potential compounds through docking analysis and validating their binding affinity and stability through molecular dynamics simulations.
BIOTECHNOLOGY PROGRESS
(2021)
Article
Medical Laboratory Technology
Giulia Brisotto et al.
Summary: The study showed that SARS-CoV-2 mRNA vaccines can induce a lasting IgG response for at least 4 months, even beyond the parameters of clinical trials. The observed antibody decay at follow-up suggests further research is needed to characterize the immune response and identify individuals with low anti-SARS-CoV-2 immunity who may require a vaccination booster.
CLINICA CHIMICA ACTA
(2021)
Article
Medical Laboratory Technology
Lin Shen et al.
Summary: In third-world countries, the lateral flow immunoassay strip (LFIA) may be a better method for detecting SARS-CoV-2, and this study developed a highly specific LFIA with a sensitivity and specificity of 98.22% and 97.93%, respectively.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Chemistry, Analytical
Malik Saddam Khan et al.
Summary: A novel and simple label-free electrochemiluminescence immunosensor was proposed using Ce2Sn2O7 nanocubes as ECL emitters, which were functionalized with gold nanoparticles to enhance ECL emission. The immunosensor showed improved ECL performance for detecting CEA concentrations, with outstanding stability, reproducibility, and specificity, making it an innovative method for sensitive analysis of biomarkers in real samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Marcelino Varona et al.
Summary: The research utilizes molecular beacon (MB) probes for sequence-specific detection of LAMP on LFIA strips, showing detection capability for various DNA sequences and single-nucleotide polymorphisms. Coupling the method with solid-phase microextraction allows for detection of target sequences in different samples, and a 3D printed device is designed to prevent contamination.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Mei Dang et al.
Summary: ATP not only biphasically modulates LLPS of the viral N protein, but also dissolves droplets induced by oligonucleic acid. Residue-specific NMR characterization showed ATP specifically binds to the RNA-binding domain (RBD) of the N protein with an average Kd of 3.3 +/- 0.4 mM.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Lei Sun et al.
Summary: Research on the structure and interactions of SARS-CoV-2 RNA with host proteins has identified potential therapeutic targets and strategies for reducing viral infection, shedding light on new avenues for COVID-19 treatment.
Article
Infectious Diseases
Anna Christine Nilsson et al.
Summary: This study evaluated six commercial serological assays for detection of SARS-CoV-2 antibodies, finding that ELISA assays are most suitable for mild cases, while Livzon LFT and Euroimmun ELISA have the highest specificity, making them suitable for seroprevalence studies.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biochemical Research Methods
Margaret A. Black et al.
Summary: The lateral flow immunoassay Biolidics 2019-nCoV IgG/IgM Detection Kit showed comparable clinical performance to ELISA in detecting SARS-CoV-2 antibodies, with high sensitivity and specificity. There was consistent performance across different sample types, suggesting the potential for decentralized rapid testing and self-testing for anti-SARS-CoV-2 antibodies.
JOURNAL OF IMMUNOLOGICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Lohra M. Miller et al.
Summary: The heterogeneity of glycosylation on the protein trimer of SARS-CoV-2 virus has been evaluated using charge detection mass spectrometry (CDMS), showing a broad distribution of glycan processing. CDMS measurements reveal significantly larger average glycan masses than those obtained from glycoproteomics studies, indicating an underestimation of the abundances of larger, more complex glycans.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Shiliang Chen et al.
Summary: The newly developed SERS-based LFIA strip using GERTs achieved highly sensitive detection of anti-SARS-CoV-2 IgM and IgG, with detection limits 100 times lower compared to conventional LFIA strips, improving the accuracy and reliability of early diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Aihua Lyu et al.
Summary: An automatic label-free chemiluminescence immunoassay (CLIA) for rapid SARS-CoV-2 nucleocapsid protein (NP) detection with high sensitivity and throughput has been developed. It integrates dualfunctionalized chemiluminescent magnetic beads, an automatic chemiluminescence analyzer, and a novel detection strategy, showing great potential for practical use in detecting SARS-CoV-2 NP.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Jing Zhang et al.
Summary: This study demonstrated the use of gold-platinum nanoflowers as colored and catalytic labels for ultrasensitive lateral flow miRNA-21 assay, achieving significantly improved sensitivity and accuracy in detection. The dual functionality of AuPt NFs allowed for detection of the lowest concentration of miRNA-21 with satisfactory results in direct testing of cell lysates. The method shows great potential for ultrasensitive lateral flow nucleic acid biosensors in on-site testing and biomedical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Kaikai Xiang et al.
Summary: Researchers synthesized silica-based nanoenzymes for rapid detection of Hg2+ concentrations, offering a simple, cost-effective, and fast response method that has been demonstrated to be practical in complex lake water.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Haifeng Liu et al.
Summary: This study reported a surface-enhanced Raman scattering-based lateral flow immunoassay method for the high sensitivity simultaneous detection of anti-SARS-CoV-2 IgM/IgG. The method utilized custom SERS tags and dual layers of Raman dye, with signal intensities easily recorded by a portable Raman instrument for high sensitivity analysis. Validation on samples from COVID-19 patients and healthy individuals showed high accuracy and specificity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Analytical
Emilie Ernst et al.
Summary: The COVID-19 pandemic has led to increased focus on clinical assays, particularly serological testing, to assess exposure to SARS-CoV-2 and immune response to the virus and vaccines. While some tests faced quality issues, they still contribute to public health efforts, highlighting challenges for both emerging technologies and established platforms.
Article
Chemistry, Analytical
Shijun Li et al.
Summary: CRISPR-top is a novel diagnostic method that integrates target pre-amplification and CRISPR-mediated detection in one reaction mixture for SARS-CoV-2 detection. This simple, rapid, accurate, and highly sensitive method can return results within 60 minutes, making it suitable for clinical and resource-challenged settings.
Review
Chemistry, Analytical
Yaofeng Zhou et al.
Summary: The global COVID-19 pandemic caused by the novel coronavirus has posed a serious threat to human life and health since its discovery in December 2019. Lateral flow assay (LFA) has been widely accepted as a critical point-of-care diagnostic platform for the rapid diagnosis of COVID-19, although it faces challenges and requires continuous improvement to enhance its efficiency in clinical diagnostics.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Qing Zhang et al.
Summary: This article discusses the application and development of optical lateral flow test strip biosensors in the field of pesticide detection, emphasizing the structure, detection principle, recent advances, advantages, potential limitations, challenges, and future trends of these biosensors in real applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Laura Jo Sherwood et al.
Summary: Researchers have rapidly generated and characterized immunoreagents using synthetic genes, recombinant antibody pipelines, and modular fusion proteins for studying novel emerging viruses. Their study with SARS-CoV-2 demonstrated the effectiveness of these immunoreagents in detecting infections and imaging, with potential applications for other coronaviruses.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xiong Zhu et al.
Summary: The study developed a novel diagnostic test for COVID-19, called COVID-19 MCCD, by combining MCDA with CRISPR-Cas12a technology, which is sensitive, rapid, and reliable for the diagnosis of SARS-CoV-2 infection.
Article
Chemistry, Multidisciplinary
Rui Chen et al.
Summary: A new serological diagnostic method was designed for early detection of COVID-19 using AIE nanoparticles. The method showed comparable or higher sensitivity to ELISA and outperformed a commercial colloidal gold nanoparticle test strip. Early detection of IgM and IgG was achieved within 1-7 days of symptom onset with the AIE nanoparticle-based test strip.
Article
Biochemistry & Molecular Biology
Naveenchandra Suryadevara et al.
Summary: The study found that a subset of human monoclonal antibodies derived from convalescent SARS-CoV-2 patients possess neutralizing activity, with two antibodies capable of inhibiting infection. Mechanistic studies revealed these antibodies neutralize in part by inhibiting post-attachment steps in the infection cycle.
Article
Chemistry, Multidisciplinary
Saptarshi Banerjee et al.
Summary: The PINAT technology is a platform for diagnosing pathogen-associated infections, offering a unified single-step, single-chamber procedure in a portable device with mobile app integration. It has demonstrated high sensitivity and specificity in various settings, making it suitable for resource-limited environments to meet the needs of underserved populations.
Article
Multidisciplinary Sciences
Erica Diani et al.
Summary: The study analyzed the diagnostic accuracy of the COVID-19 IgG/IgM rapid test cassette lateral flow immunoassay test and found that it displayed reliable performance in detecting SARS-CoV-2 infection, with a higher positivity rate observed after 10 days from symptom onset. Additionally, the LFIA assay showed valid performance compared to other LFIA assays in detecting IgM and IgG antibodies.
Article
Chemistry, Analytical
Mingyuan Zou et al.
Summary: The study reports the development of an accurate, rapid, and easy-to-implement isothermal nonenzymatic signal amplification system coupled with a lateral flow immunoassay strip-based detection method for detecting SARS-CoV-2 in oropharyngeal swab samples. This method offers a fast, simple, and sensitive alternative to the SARSCoV-2 RT-PCR assay, with 100% positive and negative predictive agreements.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Analytical
Linzhe Chen et al.
Summary: Diagnostic tools play significant roles in combating COVID-19, with emerging biosensing technologies offering rapid and highly sensitive tests, yet facing challenges in practical applications.
Review
Chemistry, Analytical
Briza Perez-Lopez et al.
Summary: The current situation of the Covid-19 pandemic is characterized by a high number of infections, high lethality, and rapid spread, severely affecting the global economy. Rapid diagnosis and testing are crucial in fighting the pandemic and maintaining public health. Various biosensing and diagnostic technologies are being developed and used to monitor the spread of the virus, with in vitro diagnosis companies striving to validate and deploy their technology quickly in the international market.
Review
Chemistry, Analytical
Jiaqi Bu et al.
Summary: SARS-COV-2 is a highly infectious novel coronavirus that has caused a global infection crisis. Detecting the virus rapidly and accurately is crucial, and surface molecular imprinting technology (SM-MIT) shows great potential with its advantages of low cost and high stability.
Article
Chemistry, Analytical
Qiaoge Bayin et al.
Summary: The study introduces a novel lateral flow immunoassay method based on superparamagnetic nanoparticles and giant magnetoresistance sensing system for simultaneous quantitative detection of anti-SARS-CoV-2 immunoglobulin M and G. This method offers advantages in cost-effectiveness, time efficiency, and ease of operation.
Review
Chemistry, Analytical
Ayushi Bisht et al.
Summary: Nanomaterial-based detection technology offers advantages in selectivity and sensitivity, making it ideal for developing portable detection kits for rapid and accurate detection of the COVID-19 virus.
JOURNAL OF ANALYSIS AND TESTING
(2021)
Review
Chemistry, Analytical
Gao-Pan Dong et al.
Summary: The outbreak of severe pneumonia caused by SARS-CoV-2 virus spread rapidly worldwide through human-to-human transmission, leading to a high number of suspected cases and massive daily testing requirements. The medical pressure increased significantly, necessitating the urgent establishment of more efficient and accurate diagnostic methods.
JOURNAL OF ANALYSIS AND TESTING
(2021)
Article
Nanoscience & Nanotechnology
Chongwen Wang et al.
Summary: A dual-mode lateral flow immunoassay biosensor was developed for high-sensitivity simultaneous detection of SARS-CoV-2 spike and nucleocapsid protein antigens, offering enhanced accuracy and efficiency in viral infection screening. The system demonstrated superior performance in detecting SARS-CoV-2 antigens in saliva and nasal swab samples, with detection limits of 1 and 0.5 pg/mL for the spike and nucleocapsid protein antigens, respectively.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemical Research Methods
Carsten Schmidt et al.
Summary: A lateral flow assay based on fluorescently encoded nanoparticles was developed for the sensitive detection of rheumatoid factor, a biomarker for rheumatoid arthritis. The assay showed high sensitivity and specificity in detecting positive and negative cases of rheumatoid arthritis through analysis of patient sera samples.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Supriya Srivastav et al.
Summary: The study compared the performance of a conventional lateral flow assay (LFA) with a surface-enhanced Raman scattering (SERS)-based LFA test in detecting SARS-CoV-2-specific IgM/IgG in sera of COVID-19 patients. The custom-built SERS reader showed at least an order of magnitude higher sensitivity than conventional LFAs with naked-eye detection. SERS detection in a purified system showed a sensitivity that is 7 orders of magnitude higher than naked-eye detection, indicating the high potential of SERS-based LFAs in point-of-care testing.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Meiting Yang et al.
Summary: A portable and accurate nucleic acid detection system was developed to address the urgent need for on-site virus screening and infection control. It utilizes ultrasensitive isothermal amplification to achieve rapid and sensitive detection of SARS-CoV-2 RNA, providing a cost-effective solution for emergency detection of the virus.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Lihong Su et al.
Summary: A dual-model colorimetric/SERS lateral flow immunoassay based on a metallic core-shell Au/Au nanostar was constructed for ultrasensitive determination of clenbuterol. The method achieved visual detection of clenbuterol at 5 ng/mL and quantitative detection as low as 0.05 ng/mL, significantly lower than traditional AuNPs-based immunoassays.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Christopher P. Mancuso et al.
Summary: Quantitative diagnostics are crucial for controlling infectious diseases and informing treatment strategies. The qRPA technology provides semiquantitative information and may be useful for monitoring the progress of viral infections or other diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Lei Huang et al.
Summary: The lateral flow assay (LFA) is a widely used paper-based on-site biosensor for detecting target analytes in minutes. Aptamers, as biorecognition molecules, are being explored as an alternative to antibodies in LFAs, offering advantages over traditional methods. Different formats of aptamer-based LFAs can achieve on-site detection of target analytes efficiently.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Ying Peng et al.
Summary: An electrochemical biosensor for sensitive monitoring of SARS-CoV-2 RNA has been proposed, with significantly amplified electrochemical signals and low detection limit, showing great prospects for clinical application in complex matrices and clinical patient samples.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Raffaella Bucci et al.
Summary: Researchers stabilized colloidal gold nanoparticles using ultrashort α,β-peptides, finding the best candidate that can stabilize GNPs in physiological media and further capped with albumin to enhance colloidal stability.
Article
Chemistry, Analytical
Zi-Tao Zhong et al.
Summary: The study combines pregnancy test strips with signal amplification technology to achieve rapid detection of various bio-molecules, and uses magnetic separation technology to achieve rapid and sensitive detection of multiple targets.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Yaofeng Zhou et al.
Summary: The study successfully developed highly luminescent quantum dot nanobeads for rapid and accurate measurement of total antibody concentrations in the population, which is crucial for combating the COVID-19 pandemic.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Multidisciplinary
Nan Jiang et al.
Summary: Readily deployable, low-cost point-of-care medical devices are urgently needed in resource-poor settings to screen various chemical and biological analytes for rapid diagnostics, benefiting public health in areas with limited access to healthcare.
Article
Chemistry, Analytical
Daniel W. Bradbury et al.
Summary: The innovative 3D printed casing incorporates lateral-flow immunoassay, dehydrated signal enhancement reagents, and a sealed buffer chamber, allowing for easy detection of the SARS-CoV-2 N-protein in undiluted serum in just 40 minutes.
Article
Chemistry, Analytical
Linlin Zhuang et al.
Summary: The ESEA-LFIA method developed in this study is a rapid and sensitive detection technology for the novel coronavirus, capable of completing the test within one hour, with good sensitivity and specificity for clinically relevant sequences.
Article
Chemistry, Analytical
Lai-Di Xu et al.
Summary: This method utilizes red emission-enhanced CD-based silica spheres to ultrasonically detect SARS-CoV-2 NP, demonstrating fast detection speed, high specificity, and ease of operation.
Article
Biochemical Research Methods
Guang Li et al.
Summary: Antibody detection in COVID-19 diagnosis offers advantages of convenience, high throughput, and low cost when combined with nucleic acid testing. Virus infection stimulates high antibody titer against SARS-CoV-2 nucleocapsid protein, which can be used to detect COVID-19 in both infected and convalescent patients.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Chemistry, Analytical
Chongwen Wang et al.
Summary: The study introduced a lateral flow immunoassay method using quantum dot nanotags for simultaneous detection of SARS-CoV-2-specific IgM and IgG in patient serum, demonstrating high sensitivity and specificity. The method showed great potential for rapid and accurate identification of infected individuals, as validated by clinical samples.
Article
Chemistry, Multidisciplinary
Guan Wang et al.
Summary: Fluorescent polymeric nanoparticles (FPPs) have attracted attention in fluorescent lateral flow assay (FLFA) for their biocompatibility, stability, and sensitivity. A novel strategy utilizing aggregation-induced emission luminogens (AIEgen) for preparing FPPs is proposed, with FPPs@210 showing optimal performance for FLFA testing using COVID-19 antibodies as targets. This study presents a promising approach for constructing FLFA strips with ideal FPPs for bioassays.
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Velu Ranganathan et al.
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Yuanjin Zhan et al.
ANALYTICAL CHEMISTRY
(2020)
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Biophysics
Van-Thuan Nguyen et al.
BIOSENSORS & BIOELECTRONICS
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Virology
Zhengtu Li et al.
JOURNAL OF MEDICAL VIROLOGY
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Muhsin Ali et al.
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Zhenhua Chen et al.
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Xiaohan Yang et al.
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Xiaoxian Liu et al.
SENSORS AND ACTUATORS B-CHEMICAL
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Biochemical Research Methods
Rashid Aman et al.
ACS SYNTHETIC BIOLOGY
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Chao Huang et al.
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Dali Wei et al.
ANALYTICA CHIMICA ACTA
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Abdu Ahmed Abdullah AL-maskri et al.
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Benjamin D. Grant et al.
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Biophysics
Yuhao Wu et al.
BIOSENSORS & BIOELECTRONICS
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Sangheon Yu et al.
ANALYTICAL CHEMISTRY
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Evgeny N. Nikolaev et al.
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Xiong Zhu et al.
BIOSENSORS & BIOELECTRONICS
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Biochemistry & Molecular Biology
Hangping Yao et al.
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Medicine, Research & Experimental
Ariane Sternberg et al.
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Cell Biology
Ya-Wen Cheng et al.
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Chemistry, Multidisciplinary
Alexander N. Baker et al.
ACS CENTRAL SCIENCE
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Muhammad Asif et al.
CURRENT OPINION IN ELECTROCHEMISTRY
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Gyanendra Bahadur Chand et al.
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Zhizeng Wang et al.
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Lu Zeng et al.
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Tian Wen et al.
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Haicong Shen et al.
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Chongwen Wang et al.
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Xi-Hui Mu et al.
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Yi Zeng et al.
APPLIED MATERIALS TODAY
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Panpan Wang et al.
ACS APPLIED NANO MATERIALS
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Di Zhang et al.
BIOSENSORS & BIOELECTRONICS
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Seungri Song et al.
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Aruni P. K. K. Karunanayake Mudiyanselage et al.
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Hongmei Zhang et al.
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Xu Yan et al.
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Hossein Abdolmohammad-Zadeh et al.
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Wei Wang et al.
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Tao Wang et al.
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