期刊
TALANTA
卷 251, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2022.123788
关键词
Electrochemiluminescence; Multiple-target detection; Bi-enzyme cascade catalysis; Peroxydisulfate; Aptasensor
As a label-free and amplified electrochemiluminescence (ECL) sensing platform, this study developed a convenient method to detect multiple targets of hemin, glucose and thrombin (TB) using peroxydisulfate (S2O82-) solution. The target-induced bi-enzyme cascade catalysis reaction was employed to enhance the ECL response strongly, leading to the nanomolar, micromolar and femtomolar detection of hemin, glucose and TB, respectively.
As various aptasensors are adopted in clinical diagnosis, the development of convenient multiple-target deteromination is a field of ever-increasing interests. Herein, a label-free and amplified electrochemiluminescence (ECL) sensing platform was constructed to detect multiple targets of hemin, glucose and thrombin (TB) using peroxydisulfate (S2O82-) solution, which was one of the most convenient and economical ECL systems. It was worth mentioning that the target-induced bi-enzyme cascade catalysis reaction was developed to increase the ECL response strongly of S2O82- solution due to the production of (O-1(2))(2)* from the inter-reaction between reactive oxygen species (ROS) and sulfate radical (SO4 center dot-). Specifically, with the layer-by-layer assembly of multi-walled carbon nanotubes (MWCNTs), glucose oxidase (GOx) and gold nanoparticles (AuNPs) as the interface, the guanine-rich (G-rich) thrombin aptamer (TBA) was anchored for hemin (target 1) detection, due to the electrocatalysis of hemin/G-quadruplex as a horseradish peroxidase mimicking DNAzyme (HRP-DNAzyme) towards dissolved oxygen for ROS generation. Second, in the presence of glucose (target 2), the ECL intensity was improved because glucose was the substrate of the bi-enzyme cascade catalysis reaction. Third, when TB (target 3) was sequentially incubated based on the above-mentioned aptasensor, the bi-enzyme catalysis was inhibited to decrease the ECL signal, due to the steric hindrance effect of the TB protein. As a result, the aptasensor achieved the nanomolar detection for hemin (3.33 nM), the micromolar detection for glucose (0.33 mu M) and the femtomolar detection for TB (3.33 fM), respectively.
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