MXene nanosheet loaded gold nanocluster catalytic amplification-aptamer SERS quantitative assay platform for isocarbophos

The traditional preparation of MXeneTi(3)C(2) is complicated. Two-dimensional MXeneTi(3)C(2) nanosheets were prepared via in-situ generation of HF etching Ti3AlC2 under the microwave irradiation. Gold nanocluster doped nanosheets sol (MXene@Au) with excellent stability and strong catalysis was synthesized with no product res-idue reducer CO. MXene@Au nanosol can catalyze mandelic acid-HAuCl4 to produce gold nanoparticles, which can be traced via resonance Rayleigh scattering (RRS) and surface-enhanced Raman scattering (SERS). Coupled the dual-mode nanocatalytic indicator reaction with aptamer reaction, a high sensitivity, selectivity, facile SERS/ RRS dual-mode biosensnoring assay platform has been constructed for isocarbophos (ICP). The linear range of SERS was 1.0 x 10(-3)-2.5 x 10(-2) nmol/L, with a detection of limit 4.5 x 10(-5) nmol/L (S/N = 3). It has been used to detect ICP in water samples, with a good recovery (95.5-104%) and a good relative standard deviation (5.2-9.6%). This new nanocatalytic amplification biosensoring strategy can also assay other organic pesticides including malathion, glyphosate, profenofos and carbendazim. In addition, the nanocatalytic mechanism was investigated.

Automated summary

Two-dimensional MXeneTi(3)C(2) nanosheets were successfully prepared by in-situ generation of HF etching Ti3AlC2 under microwave irradiation. The nanosheets sol of MXene@Au, which is doped with gold nanoclusters and has excellent stability and strong catalysis, was synthesized without CO as a product residue reducer. MXene@Au nanosol can catalyze mandelic acid-HAuCl4 to produce gold nanoparticles, which can be traced via resonance Rayleigh scattering (RRS) and surface-enhanced Raman scattering (SERS). A high sensitivity, selectivity, and facile SERS/RRS dual-mode biosensing assay platform has been constructed for isocarbophos (ICP) by coupling the dual-mode nanocatalytic indicator reaction with the aptamer reaction.