4.7 Article

One-step self-assembling multiple hydrophobic quantum dots and hydrophilic biomolecules into all-inclusive tags for ultrasensitive immunosensors

期刊

SENSORS AND ACTUATORS B-CHEMICAL
卷 373, 期 -, 页码 -

出版社

ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.132720

关键词

Hydrophobic quantum dots; Self -assemble; One-step; Multiplex immunochromatography assay; PED-5 inhibitors

资金

  1. National Scientific Foundation of China [31871883, 21874048]
  2. HeYuan Planned Program in Science and Technology [2019041]
  3. Generic Technique Innovation Team Construction of Modern Agriculture of Guangdong Province [2021KJ130]
  4. National Key Research and Development Program of Thirteenth Five-Year Plan [2017YFC1601700]
  5. Guangdong Basic and Applied Basic Research Foundation [2019B1515210025, 2020A1515011238]
  6. Science and Technology Planning Project of the Guangxi [AB18126048]

向作者/读者索取更多资源

This study introduces a novel approach to synthesize antibody-decorated vesicles with high QDs-loading, outstanding dispersity, and high quantum yields, leading to significant improvements in sensitivity and accuracy for immunoassays.
High quality hydrophobic quantum dots (QDs) are increasingly popular in developing ultrasensitive immuno-assays. However, the conjugation of biomolecules usually suffers from complex modifications and compromised luminescence. Herein, a novel one-step emulsification-assisted approach was described to synthesize antibody -decorated vesicles with high QDs-loading, outstanding dispersity and high quantum yields sustaining. Initially, the prerequisites for fabricating such vesicles were systematically investigated. QDs diluted in non-polar solvents were emulsified into compact nanospheres and accommodated with recognizing antibodies and stabi-lizing proteins that contained abundant amino groups. Then, the universality of this technique was verified by introducing three kinds of hydrophobic QDs to fabricate similar vesicles. Finally, to evaluate the applicability, an immunochromatographic assay toward 12 prohibited phosphodiesterase-5 inhibitors in health foods was developed with outstanding sensitivity (a significant analytical improvement of 5-80 folds compared to the reported ICA methods) and high accuracy. The present work highlights a novel and effective approach to produce high hydrophobic QDs-loaded immunoprobes, which is potentially generalizable to overcome limitations of weak fluorescence, low dispersity and complicated synthesis when conjugating biomolecules and hydrophobic QDs.

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