3D DNA walker recognition-driven homogeneous dual-mode sensing strategy based on enzyme biofuel cell for ultrasensitive detection of HER2

An ultrasensitive enzyme biofuel cell (EBFC) based self-powered biosensor is developed for electrochemical/ colorimetric dual-mode detection of human epidermal growth factor receptor 2 (HER2). Graphdiyne (GDY) and tetragonal molybdenum disulfide (1 T MoS2) are prepared to construct high-performance EBFC. Threedimensional (3D) DNA Walker triggers hybrid chain reaction (HCR) cascade strategy is used to further improve the output signal intensity and selectivity of assay. Furthermore, a capacitor is matched to EBFC to generate electrical signals magnified several times for boosting the analytical performance. An ultrasensitive dual-mode assay is therefore developed for HER2 with an expanded linear response range of 0.1-10000 pg/mL and a low detection limit of 0.03 pg/mL (S/N = 3). The results confirm the developed assay provides a powerful tool for ultrasensitive and accurate detection of cancer marker.

Automated summary

An ultrasensitive enzyme biofuel cell (EBFC) based self-powered biosensor is developed for dual-mode detection of HER2. Graphdiyne (GDY) and tetragonal molybdenum disulfide (1T MoS2) are used to construct high-performance EBFC. Threedimensional (3D) DNA Walker triggers hybrid chain reaction (HCR) cascade strategy is employed to improve the assay's output signal intensity and selectivity. Additionally, a capacitor is matched to EBFC for magnifying the electrical signals and boosting the analytical performance. The developed ultrasensitive dual-mode assay exhibits an expanded linear response range and low detection limit for HER2, providing a powerful tool for accurate detection of cancer marker.