Aerobic denitrifying using actinobacterial consortium: Novel denitrifying microbe and its application

The aerobic denitrifying capacity of actinomycete strain has been investigated recently, while little is known about nitrogen and carbon substrate removal by mix-cultured aerobic denitrifying actinobacteria (Mix-CADA) community. Hence, three Mix-CADA consortiums, named Y23, X21, and Y27, were isolated from urban lakes to investigate their aerobic denitrification capacity, and their removal efficiency for nitrate and dissolved organic carbon were >97 % and 90 %, respectively. Illumina Miseq sequencing revealed that Streptomyces was the most dominant genus in the Mix-CADA consortium. Network analysis indicated that Streptomyces exfoliates, as the core species in the Mix-CADA consortium, majorly contributed to dissolved organic carbon and total nitrogen reduction. Moreover, the three Mix-CADA consortiums could remove 78 % of the total nitrogen and 61 % of the permanganate index from the micro -polluted l water. Meanwhile, humic-like was significantly utilized by three Mix-CADA consortiums, whereas Mix-CADA Y27 could also utilize aromatic protein and soluble microbial by-product-like in the micro-polluted raw water purification. In summary, this study will offer a novel perspective for the purification of micro-polluted raw water using the Mix-CADA consortium.

Automated summary

This study investigated the aerobic denitrification capacity and substrate removal efficiency of three mix-cultured aerobic denitrifying actinobacteria (Mix-CADA) consortiums isolated from urban lakes. The removal efficiency for nitrate and dissolved organic carbon by the consortiums was found to be >97% and 90%, respectively. Network analysis revealed that Streptomyces played a major role in the reduction of dissolved organic carbon and total nitrogen. The study suggests that the Mix-CADA consortium could be a novel approach for the purification of micro-polluted raw water.