4.6 Article

Genetic code expansion to enable site-specific bioorthogonal labeling of functional G protein-coupled receptors in live cells

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Multidisciplinary Sciences

FRET sensors reveal the retinal entry pathway in the G protein-coupled receptor rhodopsin

He Tian et al.

Summary: In this study, Rho mutants were engineered as FRET sensors to measure the kinetics and energetics of 11CR binding using genetic code expansion and bioorthogonal labeling. The study found that mutations in the channel between transmembrane helices 5 and 6 dramatically affected 11CR binding kinetics, but not agonist release kinetics. The findings provided direct experimental evidence for the entry of 11CR between TM5/6 in Rho, involving dynamic allosteric control of the ligand entry channel. These findings proposed a conceptual framework for understanding the function of G protein-coupled receptors with hydrophobic ligands that enter their binding pockets through transmembrane pores.

ISCIENCE (2022)

Article Chemistry, Multidisciplinary

A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1

Xiaoyan Wang et al.

Summary: A method for genetic incorporation of an unnatural amino acid into a cannabinoid receptor has been developed, revealing a previously unrecognized pre-active state through F-19 NMR and X-ray crystallography methods. An allosteric modulation model is proposed to explain the distinct behavior of the allosteric modulator Org27569. This site-specific F-19 NMR labeling method is a powerful tool for decoding the mechanism of GPCR allosteric modulation.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

Article Multidisciplinary Sciences

Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain

Maria Kowalski-Jahn et al.

Summary: The study explores the conformational effects of different WNT proteins on FZD receptors, revealing that CRD dynamics are not essential for WNT/beta-catenin signaling.

SCIENCE ADVANCES (2021)

Article Biochemistry & Molecular Biology

Genetic code expansion in mammalian cells: A plasmid system comparison

Wenyuan Zhou et al.

BIOORGANIC & MEDICINAL CHEMISTRY (2020)

Article Biochemistry & Molecular Biology

Quantitative Single-Residue Bioorthogonal Labeling of G Protein-Coupled Receptors in Live Cells

Robert Serfling et al.

ACS CHEMICAL BIOLOGY (2019)

Article Biochemistry & Molecular Biology

Genetic code expansion and photocross-linking identify different β-arrestin binding modes to the angiotensin II type 1 receptor

Laurence Gagnon et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2019)

Article Biochemistry & Molecular Biology

Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells

Robert Serfling et al.

NUCLEIC ACIDS RESEARCH (2018)

Article Biochemistry & Molecular Biology

G protein subtype-specific signaling bias in a series of CCR5 chemokine analogs

Emily Lorenzen et al.

SCIENCE SIGNALING (2018)

Review Chemistry, Multidisciplinary

Labeling and Single-Molecule Methods To Monitor G Protein-Coupled Receptor Dynamics

He Tian et al.

CHEMICAL REVIEWS (2017)

Review Chemistry, Multidisciplinary

Strain-Promoted 1,3-Dipolar Cycloaddition of Cycloalkynes and Organic Azides

Jan Dommerholt et al.

TOPICS IN CURRENT CHEMISTRY (2016)

Article Pharmacology & Pharmacy

CXC Chemokine Receptor 3 Alternative Splice Variants Selectively Activate Different Signaling Pathways

Yamina A. Berchiche et al.

MOLECULAR PHARMACOLOGY (2016)

Review Biochemistry & Molecular Biology

Chemical Biology Methods for Investigating G Protein-Coupled Receptor Signaling

Thomas Huber et al.

CHEMISTRY & BIOLOGY (2014)

Article Biochemistry & Molecular Biology

Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

Mary P. Hall et al.

ACS CHEMICAL BIOLOGY (2012)

Article Biochemistry & Molecular Biology

Genetically Encoded Photo-cross-linkers Map the Binding Site of an Allosteric Drug on a G Protein-Coupled Receptor

Amy Grunbeck et al.

ACS CHEMICAL BIOLOGY (2012)

Article Biochemical Research Methods

Preventing Thiol-Yne Addition Improves the Specificity of Strain-Promoted Azide-Alkyne Cycloaddition

Remon van Geel et al.

BIOCONJUGATE CHEMISTRY (2012)

Article Biochemistry & Molecular Biology

Direct Measurement of Thermal Stability of Expressed CCR5 and Stabilization by Small Molecule Ligands

Adam M. Knepp et al.

BIOCHEMISTRY (2011)

Article Chemistry, Multidisciplinary

Readily Accessible Bicyclononynes for Bioorthogonal Labeling and Three-Dimensional Imaging of Living Cells

Jan Dommerholt et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2010)

Article Biochemistry & Molecular Biology

FTIR analysis of GPCR activation using azido probes

Shixin Ye et al.

NATURE CHEMICAL BIOLOGY (2009)

Article Biochemistry & Molecular Biology

Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment

Li-Yin Huang et al.

BIOCHEMISTRY (2008)

Article Biochemical Research Methods

Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection

Sung Ho Park et al.

JOURNAL OF IMMUNOLOGICAL METHODS (2008)

Article Chemistry, Multidisciplinary

Tetrazine ligation: Fast bioconjugation based on inverse-electron-demand Diels-Alder reactivity

Melissa L. Blackman et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)

Review Biochemical Research Methods

Bioluminescent assays for high-throughput screening

Frank Fan et al.

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES (2007)

Article Chemistry, Multidisciplinary

A strain-promoted [3+2] azide-alkyne cycloaddition for covalent modification of blomolecules in living systems

NJ Agard et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)

Article Multidisciplinary Sciences

An expanded eukaryotic genetic code

JW Chin et al.

SCIENCE (2003)

Article Biochemistry & Molecular Biology

Progress toward an expanded eukaryotic genetic code

JW Chin et al.

CHEMISTRY & BIOLOGY (2003)

Article Biochemistry & Molecular Biology

A general approach for the generation of orthogonal tRNAs

L Wang et al.

CHEMISTRY & BIOLOGY (2001)

Article Multidisciplinary Sciences

Expanding the genetic code of Escherichia coli

L Wang et al.

SCIENCE (2001)