期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2209884119
关键词
cone photoreceptor; color vision; thyroid hormone receptor; THRB; retina
资金
- NIDDK at the National Institutes of Health
Cone photoreceptor diversity is crucial for color vision, but little is known about the control of diversity in these neurons. In this study, the researchers derived a Cre driver to isolate individual M and S opsin-enriched cones in mice and identified expression gradients for groups of genes. They found that thyroid hormone receptor ss 2 (TR ss 2) plays a role in controlling the expression of these genes and promoting cone diversity.
Cone photoreceptor diversity allows detection of wavelength information in light, the first step in color (chromatic) vision. In most mammals, cones express opsin photopigments for sensitivity to medium/long (M, green) or short (S, blue) wavelengths and are differentially arrayed over the retina. Cones appear early in retinal neurogenesis but little is understood of the subsequent control of diversity of these postmitotic neurons, because cone populations are sparse and, apart from opsins, poorly defined. It is also a challenge to distinguish potentially subtle differences between cell subtypes within a lineage. Therefore, we derived a Cre driver to isolate individual M and S opsin-enriched cones, which are distributed in counter-gradients over the mouse retina. Fine resolution transcriptome analyses identified expression gradients for groups of genes. The postnatal emergence of gradients indicated divergent differentiation of cone precursors during maturation. Using genetic tagging, we demonstrated a role for thyroid hormone receptor ss 2 (TR ss 2) in control of gradient genes, many of which are enriched for TR ss 2 binding sites and TR ss 2-regulated open chromatin. Deletion of TR ss 2 resulted in poorly distinguished cones regardless of retinal location. We suggest that TR ss 2 controls a bipotential transcriptional state to promote cone diversity and the chromatic potential of the species.
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