期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2207660119
关键词
spermatogenesis; sexual dimorphism; Dynein ;germline; cell cycle
资金
- CNRS, INSERM, College de France
- Fondation pour la Recherche Medicale (FRM) [Equipe FRM DEQ20160334884]
- Agence nationale de la recherche [ANR-13-BSV2-0007-02 PlasTiSiPi, ANR-15-CE13-0001-01]
- Bettencourt-Schueller Foundation (Fondation Schlumberger pour l'Education et la Recherche)
- Agence Nationale de la Recherche (ANR) [ANR-15-CE13-0001] Funding Source: Agence Nationale de la Recherche (ANR)
In Drosophila males, although meiosis differs significantly from females, similar molecular mechanisms are used to ensure proper pairing of centromeres.
In the early stages of meiosis, maternal and paternal chromosomes pair with their homologous partner and recombine to ensure exchange of genetic information and proper segregation. These events can vary drastically between species and between males and females of the same species. In Drosophila, in contrast to females, males do not form synaptonemal complexes (SCs), do not recombine, and have no crossing over; yet, males are able to segregate their chromosomes properly. Here, we investigated the early steps of homolog pairing in Drosophila males. We found that homolog centromeres are not paired in germline stem cells (GSCs) and become paired in the mitotic region before meiotic entry, similarly to females. Surprisingly, male germline cells express SC proteins, which localize to centromeres and promote pairing. We further found that the SUN/KASH (LINC) complex and microtubules are required for homolog pairing as in females. Chromosome movements in males, however, are much slower than in females and we demonstrate that this slow dynamic is compensated in males by having longer cell cycles. In agreement, slowing down cell cycles was sufficient to rescue pairing-defective mutants in female meiosis. Our results demonstrate that although meiosis differs significantly between males and females, sex-specific cell cycle kinetics integrate similar molecular mechanisms to achieve proper centromere pairing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据