Thermo- and pH-responsive poly[(diethylene glycol methyl ether methacrylate)-co-(2-diisopropylamino ethyl methacrylate)] hyperbranched copolymers: self-assembly and drug-loading

We present the synthesis of a new series of thermo- and pH-responsive poly[(diethylene glycol methyl ether methacrylate)-co-(2-diisopropylamino ethyl methacrylate)], P(DEGMA-co-DIPAEMA), hyperbranched copolymers and studies regarding their self-assembly in aqueous media. Three P(DEGMA-co-DIPAEMA) hyperbranched copolymers, with different ratios of hydrophobic to hydrophilic segments, are synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization using the branching agent ethylene glycol dimethacrylate (EGDMA). By employing several physicochemical characterization techniques, it was possible to determine the properties of self-assembled nanostructures, which are responsive to changes in aqueous solution temperature and pH. Dynamic light scattering (DLS) verified the formation of nano-aggregates in aqueous media, in particular at pH 3 and at both low and high temperatures (25 degrees C, 55 degrees C), where all hyperbranched copolymers form the largest aggregates compared to the other two pH conditions investigated (i.e., pH 7 and pH 10). In addition, the hyperbranched copolymers were able to encapsulate the hydrophobic drug indomethacin (IND) at different loading ratios. IND release experiments were performed at pH 7, and revealed that a high amount of the entrapped IND is strongly attached to the hydrophobic domains of the hyperbranched copolymer aggregates. The acquired data are encouraging for the potential of these novel hyperbranched copolymers to be used as nanocarrier systems for medicinal applications.

Automated summary

We synthesized a new series of thermo- and pH-responsive hyperbranched copolymers and studied their self-assembly in aqueous media. The copolymers showed the formation of nano-aggregates responsive to changes in temperature and pH. Encapsulation experiments with a hydrophobic drug also showed promising results. These hyperbranched copolymers have potential as nanocarrier systems for medicinal applications.