Untargeted metabolomics confirms the association between plasma branched chain amino acids and residual feed intake in beef heifers

This study explored plasma biomarkers and metabolic pathways underlying feed efficiency measured as residual feed intake (RFI) in Charolais heifers. A total of 48 RFI extreme individuals (High-RFI, n = 24; Low-RFI, n = 24) were selected from a population of 142 heifers for classical plasma metabolite and hormone quantification and plasma metabolomic profiling through untargeted LC-MS. Most efficient heifers (Low-RFI) had greater (P = 0.03) plasma concentrations of IGF-1 and tended to have (P = 0.06) a lower back fat depth compared to least efficient heifers. However, no changes were noted (P >= 0.10) for plasma concentrations of glucose, insulin, non-esterified fatty acids, beta-hydroxybutyrate and urea. The plasma metabolomic dataset comprised 3,457 ions with none significantly differing between RFI classes after false discovery rate correction (FDR > 0.10). Among the 101 ions having a raw P < 0.05 for the RFI effect, 13 were putatively annotated by using internal databases and 6 compounds were further confirmed with standards. Metabolic pathway analysis from these 6 confirmed compounds revealed that the branched chain amino acid metabolism was significantly (FDR < 0.05) impacted by the RFI classes. Our results confirmed for the first time in beef heifers previous findings obtained in male beef cattle and pointing to changes in branched-chain amino acids metabolism along with that of body composition as biological mechanisms related to RFI. Further studies are warranted to ascertain whether there is a cause-and-effect relationship between these mechanisms and RFI.

Automated summary

This study investigated plasma biomarkers and metabolic pathways related to residual feed intake (RFI) in Charolais heifers, finding that plasma concentrations of IGF-1 and back fat depth were associated with RFI. However, there were no changes in other plasma concentrations. Metabolic pathway analysis revealed that branched chain amino acid metabolism was significantly impacted by RFI. These findings confirm previous research in male beef cattle and suggest that changes in branched-chain amino acids metabolism and body composition are biological mechanisms related to RFI.