Carriage of three plasmids in a single human clinical isolate of Clostridioides difficile

A subset of clinical isolates of Clostridioides difficile contains one or more plasmids and these plasmids can harbor virulence and antimicrobial resistance determinants. Despite their potential importance, C. difficile plasmids remain poorly characterized. Here, we provide the complete genome sequence of a human clinical isolate that carries three high-copy number plasmids from three different plasmid families that are therefore compatible. For two of these, we identify a region capable of sustaining plasmid replication in C. difficile that is also compatible with the plasmid pCD630 that is found in many laboratory strains. Together, our data advance our understanding of C. difficile plasmid biology.

Automated summary

A subset of clinical isolates of Clostridioides difficile contains plasmids that can carry virulence and antimicrobial resistance genes. However, C. difficile plasmids are poorly studied. Here, we provide the complete genome sequence of a clinical isolate carrying three high-copy number plasmids from different families, advancing our understanding of C. difficile plasmid biology.