Exogenous nano-silicon application improves ion homeostasis, osmolyte accumulation and palliates oxidative stress in Lens culinaris under NaCl stress

Lentil is one of the highly nutritious legumes but is highly susceptible to salinity stress. Silicon has been known to reduce the effect of various environmental stresses including salinity. Moreover, silicon when applied in its nano -form is expected to augment the beneficial attributes of silicon. However, very little is known regarding the prospect of nano-silicon (nSi) application for alleviating the effect of salinity stress in non-silicified plants like lentil. In this study, the primary objective was to evaluate the efficacy of nSi in the alleviation of NaCl stress during germination and early vegetative stages. In this context, different concentrations of nSi (0, 1, 5, 10 g L-1) was applied along with four different concentrations of NaCl (0, 100, 200, 300 mM). The results indicated the uptake of nSi which was confirmed by the better accumulation of silica in the plant tissues. Most importantly, the enhanced accumulation of silica increased the K+/Na+ ratio of the NaCl-stressed seedlings. Moreover, nSi effi-ciently improved germination, growth, photosynthetic pigments, and osmotic balance. On the other hand, the relatively reduced activities of antioxidative enzymes were surmounted by the higher activity of non-enzymatic antioxidants which mainly scavenged the increased ROS. Reduced ROS accumulation in return ensured better membrane integrity and reduced electrolyte leakage up on nSi application. Therefore, it can be concluded that the application of nSi (more specifically at 10 g L-1) facilitated the uptake of silica and improved the K+/Na+ ratio to reclaim the growth and physiological status of NaCl-stressed seedlings.

Automated summary

The study evaluated the efficacy of nSi in alleviating NaCl stress during germination and early vegetative stages in lentil. The results showed that nSi application improved germination, growth, photosynthetic pigments, increased antioxidative enzyme activity, reduced ROS accumulation, and ensured better membrane integrity.