期刊
PLANT PHYSIOLOGY
卷 191, 期 3, 页码 1561-1573出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/plphys/kiad018
关键词
-
This study conducted a GWAS using phenotypic data of temperate japonica rice varieties from a public database, and demonstrated the reliability and effectiveness of using legacy data for GWASs. The results successfully detected known agriculturally important genes and reliable QTL for agronomically important traits. It also proposed the use of legacy data as a strong alternative to replicate tests and to evaluate the pleiotropic effect of QTL.
Genome-wide association studies (GWASs) are used to detect quantitative trait loci (QTL) using genomic and phenotypic data as inputs. While genomic data are obtained with high throughput and low cost, obtaining phenotypic data requires a large amount of effort and time. In past breeding programs, researchers and breeders have conducted a large number of phenotypic surveys and accumulated results as legacy data. In this study, we conducted a GWAS using phenotypic data of temperate japonica rice (Oryza sativa) varieties from a public database. The GWAS using the legacy data detected several known agriculturally important genes, indicating reliability of the legacy data for GWAS. By comparing the GWAS using legacy data (L-GWAS) and a GWAS using phenotypic data that we measured (M-GWAS), we detected reliable QTL for agronomically important traits. These results suggest that an L-GWAS is a strong alternative to replicate tests to confirm the reproducibility of QTL detected by an M-GWAS. In addition, because legacy data have often been accumulated for many traits, it is possible to evaluate the pleiotropic effect of the QTL identified for the specific trait that we focused on with respect to various other traits. This study demonstrates the effectiveness of using legacy data for GWASs and proposes the use of legacy data to accelerate genomic breeding.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据