Hydrogen sulfide upregulates the alternative respiratory pathway in mangrove plant Avicennia marina to attenuate waterlogging-induced oxidative stress and mitochondrial damage in a calcium-dependent manner

Hydrogen sulfide (H2S) is considered to mediate plant growth and development. However, whether H2S regulates the adaptation of mangrove plant to intertidal flooding habitats is not well understood. In this study, sodium hydrosulfide (NaHS) was used as an H2S donor to investigate the effect of H2S on the responses of mangrove plant Avicennia marina to waterlogging. The results showed that 24-h waterlogging increased reactive oxygen species (ROS) and cell death in roots. Excessive mitochondrial ROS accumulation is highly oxidative and leads to mitochondrial structural and functional damage. However, the application of NaHS counteracted the oxidative damage caused by waterlogging. The mitochondrial ROS production was reduced by H2S through increasing the expressions of the alternative oxidase genes and increasing the proportion of alternative respiratory pathway in the total mitochondrial respiration. Secondly, H2S enhanced the capacity of the antioxidant system. Meanwhile, H2S induced Ca2+ influx and activated the expression of intracellular Ca2+-sensing-related genes. In addition, the alleviating effect of H2S on waterlogging can be reversed by Ca2+ chelator and Ca2+ channel blockers. In conclusion, this study provides the first evidence to explain the role of H2S in waterlogging adaptation in mangrove plants from the mitochondrial aspect.

Automated summary

This study investigated the effect of hydrogen sulfide (H2S) on the adaptation of mangrove plant Avicennia marina to waterlogging. The results showed that waterlogging caused oxidative damage in the roots, but the application of H2S counteracted the damage. H2S reduced mitochondrial ROS production by increasing the expressions of alternative oxidase genes and altering mitochondrial respiration. In addition, H2S enhanced the antioxidant system and activated intracellular calcium sensing. This study provides evidence for the role of H2S in waterlogging adaptation in mangrove plants from a mitochondrial perspective.