Lanostane triterpenoids from the fungus Physisporinus vitreus and their inhibitory activity against nitric oxide production

Eight undescribed lanostane triterpenoids, physivitrins A???H, along with four known analogues, were isolated from cultures of the fungus Physisporinus vitreus. Their structures were elucidated on the basis of extensive spectroscopic methods, in which the absolute configuration of physivitrin A was elucidated using electronic circular dichroism calculation and nuclear magnetic resonance (NMR) calculation with DP4+ analysis. Phys-ivitrins B and C showed inhibitory activities against nitric oxide (NO) production in LPS-activated RAW264.7 macrophages with IC50 values of 7.5 and 23.5 ??M, respectively. Meanwhile, proinflammatory cytokines (TNF-??, iNOS and IL-1??) mRNA expression was also inhibited by physivitrin B significantly.

Automated summary

Eight undescribed lanostane triterpenoids, physivitrins A-H, and four known analogues were isolated from cultures of the fungus Physisporinus vitreus. Their structures were determined using extensive spectroscopic methods, and the absolute configuration of physivitrin A was elucidated through electronic circular dichroism calculation and NMR calculation with DP4+ analysis. Physivitrins B and C showed inhibitory activities against nitric oxide production in LPS-activated RAW264.7 macrophages, with IC50 values of 7.5 and 23.5 μM, respectively. Moreover, physivitrin B significantly inhibited the mRNA expression of proinflammatory cytokines (TNF-α, iNOS, and IL-1β).