期刊
PHOTOCHEMISTRY AND PHOTOBIOLOGY
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1111/php.13751
关键词
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资金
- President Foundation of Shenzhen Stomatology Hospital [2021A001]
- Oral infectious disease mechanism research and clinical translation application innovation team of Guangdong Province of China [2021KCXTD033]
Photobiomodulation (PBM) has been shown to enhance the regenerative capacity of stem cells from human exfoliated deciduous teeth (SHEDs) for dentin regeneration, possibly through the cross talk between BMP/Smad and Wnt/β-catenin signaling pathways.
Increasing evidence suggests stem cells from human exfoliated deciduous teeth (SHEDs) serve as desirable sources of dentin regeneration. Photobiomodulation (PBM) has shown great potential in enhancing the proliferation and osteogenesis of human bone marrow mesenchymal stem cells (hBMMSCs). However, the specific role of PBM in odontogenic differentiation of SHEDs is little know, and we further investigated potential mechanism of PBM osteo/odontogenisis. A 980 nm diode laser with different energy densities of (0.5, 5, 10 J cm(-2)) in a 100-mW continuous wave was used for irradiation every 24 h. Osteo/odontogenic differentiation of SHEDs was achieved by performing alkaline phosphatase (ALP) and alizarin red staining (ARS) and osteo/odontogenic markers were also evaluated by qRT-PCR and western blotting. Additionally, western blot and immunohistochemical staining were performed to evaluate the levels of BMP/beta Smad and Wnt/beta-catenin signaling-related proteins. We found that PBM at 5 J cm(-1) increased mineral deposition and upregulated the expression of related osteo/odontogenic markers along with the elevated expression of beta-catenin and phosphorylation level of Smad1/5/9. Furthermore, Wnt signaling inhibition using DKK1 and BMP signaling inhibition using noggin inhibited PBM-induced osteo/odontogenic marker expression when used individually or jointly. In conclusion, PBM induces the osteo/odontogenic differentiation of SHEDs through cross talk between BMP/Smad and Wnt/beta-catenin signaling pathways.
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