Da-Yuan-Yin decoction polyphenol fraction attenuates acute lung injury induced by lipopolysaccharide

Context: Da-Yuan-Yin is a Chinese traditional prescription.Objective: This study explores the therapeutic effects of the Da-Yuan-Yin decoction polyphenol fraction (DYY-4) on acute lung injury (ALI) in mice induced by lipopolysaccharide (LPS).Materials and methods: The mice (n 1/410) were orally administrated with DYY-4 (15, 30, and 60 mg/kg) or DXM (5 mg/kg), half an hour after LPS (2 mg/kg) instilled intratracheally. The protein content and the levels of inflammatory factors, the levels of complements, the mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), the level of myeloperoxidase (MPO) and superoxide dis-mutase (SOD), the expression of the IkB kinase (IKK) and nuclear factor-kappa B (NF-kappa B), the lung wet-to-dry weight (W/D) ratio and lung tissue were evaluated, 24 h after LPS challenge. Network pharmacology predicted potential targets.Results: DYY-4 (30, 60 mg/kg, p < 0.01, p < 0.01) decreased the lung W/D ratio, total protein concentra-tion, the levels of C3, C3c and C5a, the levels of TNF-alpha, IL-6, and IL-1 beta, while increased the levels of IL-4 and IL-10. DYY-4 (60 mg/kg) decreased the levels of C5aR1, C5b-9 and COX-2 mRNA (p < 0.05), the levels of MPO and iNOS mRNA, the activation of the IKK/NF-jB pathway (p < 0.01), and increased the levels of IL-13 and SOD (p < 0.01). DYY-4 (60 mg/kg) relieved the lung tissue pathological changes and reduced the C3c deposition.Discussion and conclusions: Network pharmacology combined with animal experiments revealed the targets of DYY-4 alleviating ALI.

Automated summary

This study investigates the therapeutic effects of Da-Yuan-Yin on acute lung injury (ALI) and reveals its potential targets through network pharmacology combined with animal experiments.