期刊
PHARMACEUTICAL BIOLOGY
卷 61, 期 1, 页码 89-99出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2022.2157018
关键词
Seminal plasma; CUMS; apoptosis; Nrf2; TyrPho
This study investigates the preventive effects of Thai Mucuna pruriens (T-MP) seed extract against seminal vesicle damage, apoptotic and Nrf2 protein expression in mice under chronic unpredictable mild stress (CUMS). The results show that T-MP extract can improve seminal epithelial damage and secretion of fluid containing essential substances and proteins in CUMS mice, increase the expression of AR and TyrPho proteins, inhibit seminal vesicular apoptosis through the suppression of Hsp70 and caspase expression, and increase Nrf2 expression. This study demonstrates that T-MP seeds have antiapoptotic properties and can be used to enhance seminal plasma quality.
Context Thai Mucuna pruriens (L.) DC. var. pruriens (Fabaceae) or T-MP seed extract has been shown to improve sexual performance and sperm quality. Objective This study investigates the preventive effects of T-MP against seminal vesicle damage, apoptotic and Nrf2 protein expression in mice under chronic unpredictable mild stress (CUMS). Materials and methods Forty-eight male ICR mice were divided into four groups: control, CUMS, T-MP300 + CUMS and T-MP600 + CUMS. Mice in control and CUMS groups received distilled water, while those in treated groups were pretreated with T-MP extract (300 or 600 mg/kg BW) for 14 consecutive days. The CMUS and co-treated groups were exposed to one random stressor (of 12 total) each day for 43 days. Components and histopathology of the seminal vesicle were examined, along with localization of androgen receptor (AR) and caspase 3. Expression of seminal AR, tyrosine phosphorylated (TyrPho), heat shock protein 70 (Hsp70), caspases (3 and 9) and nuclear factor erythroid 2-related factor 2 (Nrf2) proteins was investigated. Results T-MP extract at a dose of 600 mg/kg BW improved seminal epithelial damage and secretion of fluid containing essential substances and proteins in CUMS mice. It also increased the expression of AR and TyrPho proteins. Additionally, T-MP increased expression of Nrf2 and inhibited seminal vesicular apoptosis through the suppression of Hsp70 and caspase expression. Conclusion T-MP seeds have an antiapoptotic property in chronic stress seminal vesicle. It is possible to apply this extract for the enhancement of seminal plasma quality.
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