期刊
CELL
卷 163, 期 6, 页码 1333-1347出版社
CELL PRESS
DOI: 10.1016/j.cell.2015.10.066
关键词
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资金
- Caenorhabditis Genetics Center (CGC) of the NIH Office of Research Infrastructure Programs [P40 OD010440]
- FP7 Marie Curie Network Nucleosome 4D
- SNSF NCCR Frontiers in Genetics
- FMI
- SNSF
Interphase chromatin is organized in distinct nuclear sub-compartments, reflecting its degree of compaction and transcriptional status. In Caenorhabditis elegans embryos, H3K9 methylation is necessary to silence and to anchor repeat-rich heterochromatin at the nuclear periphery. In a screen for perinuclear anchors of heterochromatin, we identified a previously uncharacterized C. elegans chromodomain protein, CEC-4. CEC-4 binds preferentially mono-, di-, or tri-methylated H3K9 and localizes at the nuclear envelope independently of H3K9 methylation and nuclear lamin. CEC-4 is necessary for endogenous heterochromatin anchoring, but not for transcriptional repression, in contrast to other known H3K9 methyl-binders in worms, which mediate gene repression but not perinuclear anchoring. When we ectopically induce a muscle differentiation program in embryos, cec-4 mutants fail to commit fully to muscle cell fate. This suggests that perinuclear sequestration of chromatin during development helps restrict cell differentiation programs by stabilizing commitment to a specific cell fate.
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