4.1 Article

Synthesis of 5'-fluorophosphate-modified short-interfering RNAs

期刊

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
卷 42, 期 5, 页码 358-370

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/15257770.2022.2148693

关键词

Phosphofluoridate; RNA; solid phase synthesis; nucleic acid chemistry

向作者/读者索取更多资源

This article presents an improved scheme for synthesizing a mono-fluorinated phosphoramidite for nucleic acid modification. The purification, reaction, and biological activity of the modified strands are evaluated.
We have developed an improved scheme for the synthesis of a mono-fluorinated phosphoramidite for the 5'-modification of nucleic acids using standard phosphoramidite chemistry. We describe the first report of a phosphofluoridate modified siRNA strand and have evaluated C18 HPLC for purification of modified strands from unreacted siRNA strands. Lastly, the biological activity of the high purity siRNA strands, when placed on the sense and/or antisense strand, was evaluated to assess the impact of 5' phosphofluoridate modifications on siRNA activity.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.1
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据