期刊
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
卷 42, 期 5, 页码 358-370出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15257770.2022.2148693
关键词
Phosphofluoridate; RNA; solid phase synthesis; nucleic acid chemistry
This article presents an improved scheme for synthesizing a mono-fluorinated phosphoramidite for nucleic acid modification. The purification, reaction, and biological activity of the modified strands are evaluated.
We have developed an improved scheme for the synthesis of a mono-fluorinated phosphoramidite for the 5'-modification of nucleic acids using standard phosphoramidite chemistry. We describe the first report of a phosphofluoridate modified siRNA strand and have evaluated C18 HPLC for purification of modified strands from unreacted siRNA strands. Lastly, the biological activity of the high purity siRNA strands, when placed on the sense and/or antisense strand, was evaluated to assess the impact of 5' phosphofluoridate modifications on siRNA activity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据