期刊
NUCLEIC ACIDS RESEARCH
卷 51, 期 2, 页码 831-851出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkac1206
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RNA-binding proteins (RBPs) control RNA metabolism through protein-RNA and protein-protein interactions. In this study, we identified in vivo RNA crosslinks in nuclear mRNP components and functionally analyzed the Npl3 protein. Mutations in the RNA recognition motifs (RRMs) and linker region of Npl3 revealed distinct functions of different RNA-binding domains. Importantly, a linker mutation impaired mRNP assembly, revealing a previously unknown function of Npl3. This integrative analysis can be applied to study RBPs in other RNA metabolic processes.
RNA-binding proteins (RBPs) control every RNA metabolic process by multiple protein-RNA and protein-protein interactions. Their roles have largely been analyzed by crude mutations, which abrogate multiple functions at once and likely impact the structural integrity of the large ribonucleoprotein particles (RNPs) these proteins function in. Using UV-induced RNA-protein crosslinking of entire cells, protein complex purification and mass spectrometric analysis, we identified >100 in vivo RNA crosslinks in 16 nuclear mRNP components in Saccharomyces cerevisiae. For functional analysis, we chose Npl3, which displayed crosslinks in its two RNA recognition motifs (RRMs) and in the connecting flexible linker region. Both RRM domains and the linker uniquely contribute to RNA recognition as revealed by NMR and structural analyses. Interestingly, mutations in these regions cause different phenotypes, indicating distinct functions of the different RNA-binding domains. Notably, an npl3-Linker mutation strongly impairs recruitment of several mRNP components to chromatin and incorporation of other mRNP components into nuclear mRNPs, establishing a so far unknown function of Npl3 in nuclear mRNP assembly. Taken together, our integrative analysis uncovers a specific function of the RNA-binding activity of the nuclear mRNP component Npl3. This approach can be readily applied to RBPs in any RNA metabolic process.
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