MGnify: the microbiome sequence data analysis resource in 2023

The MGnify platform (https://www.ebi.ac.uk/metagenomics) facilitates the assembly, analysis and archiving of microbiome-derived nucleic acid sequences. The platform provides access to taxonomic assignments and functional annotations for nearly half a million analyses covering metabarcoding, metatranscriptomic, and metagenomic datasets, which are derived from a wide range of different environments. Over the past 3 years, MGnify has not only grown in terms of the number of datasets contained but also increased the breadth of analyses provided, such as the analysis of long-read sequences. The MGnify protein database now exceeds 2.4 billion non-redundant sequences predicted from metagenomic assemblies. This collection is now organised into a relational database making it possible to understand the genomic context of the protein through navigation back to the source assembly and sample metadata, marking a major improvement. To extend beyond the functional annotations already provided in MGnify, we have applied deep learning-based annotation methods. The technology underlying MGnify's Application Programming Interface (API) and website has been upgraded, and we have enabled the ability to perform downstream analysis of the MGnify data through the introduction of a coupled Jupyter Lab environment.

Automated summary

The MGnify platform is a resource for analyzing and storing microbiome-derived nucleic acid sequences. It offers access to taxonomic assignments and functional annotations for a large number of datasets derived from different environments. The platform has expanded in terms of dataset quantity and analysis capabilities over the past three years, and includes a relational database for understanding the genomic context of proteins. Deep learning-based annotation methods have also been implemented to enhance functional annotations. Additionally, the platform's technology has been upgraded, and a Jupyter Lab environment has been introduced for downstream analysis of the data.