期刊
NUCLEIC ACIDS RESEARCH
卷 50, 期 22, 页码 12913-12923出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkac1151
关键词
-
资金
- National Key R&D Program of China [2022YFC3400402]
- National Natural Science Foundation of China [32200496, 32071218]
- CAS Pioneer Hundred Talents Program
- Open Project Funding of the State Key Laboratory of Biocatalysis and Enzyme Engineering [SKLBEE2021004]
The type III-E CRISPR-Cas systems are newly discovered adaptive immune systems in prokaryotes that can cleave target RNA using a single Cas7-11 protein. This study demonstrated the functional linkage between the RNase and protease activities in type III-E systems and elucidated the mechanisms underlying the target RNA-guided proteolytic activity of Csx29. The findings will guide further developments leveraging this simple RNA targeting system for RNA and protein-related applications.
The type III-E CRISPR-Cas systems are newly identified adaptive immune systems in prokaryotes that use a single Cas7-11 protein to specifically cleave target RNA. Cas7-11 could associate with Csx29, a putative caspase-like protein encoded by the gene frequently found in the type III-E loci, suggesting a functional linkage between the RNase and protease activities in type III-E systems. Here, we demonstrated that target RNA recognition would stimulate the proteolytic activity of Csx29, and protein Csx30 is the endogenous substrate. More interestingly, while the cognate target RNA recognition would activate Csx29, non-cognate target RNA with the complementary 3 ' anti-tag sequence inhibits the enzymatic activity. Csx30 could bind to the sigma factor RpoE, which may initiate the stress response after proteolytic cleavage. Combined with biochemical and structural studies, we have elucidated the mechanisms underlying the target RNA-guided proteolytic activity of Csx29. Our work will guide further developments leveraging this simple RNA targeting system for RNA and protein-related applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据