4.6 Article

Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids

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A high-efficiency method for site-directed mutagenesis of large plasmids based on large DNA fragment amplification and recombinational ligation

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Summary: The study introduces an effective method, SMLP, for site-directed mutagenesis in large plasmids based on PCR techniques. The SMLP method showed advantages over traditional methods in mutagenesis of large plasmids, offering simplicity and effectiveness for laboratories working with large plasmids.

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