MicroRNA152-3p Protects Against Ischemia/Reperfusion-Induced Bbb Destruction Possibly Targeting the MAP3K2/JNK/c-Jun Pathway

In the current study, we reported that overexpression of miR-152-3p effectively ameliorated neurological deficits and protected blood-brain barrier(BBB) integrity in middle cerebral artery occlusion (MCAO) rats. In an in vitro model, the level of miR-152-3p was significantly decreased in bEnd.3 cells after oxygen-glucose deprivation/reperfusion (OGD/R) insult. miR-152-3p overexpressing bEnd.3 cell monolayers were protected from OGD/R-induced microvascular hyperpermeability. The miR-152-3p-mediated protective effect was associated with lower apoptosis of endothelia by negatively modulating the MAP3K2/JNK/c-Jun pathway.

Automated summary

The study showed that overexpression of miR-152-3p improved neurological deficits and protected blood-brain barrier integrity in rats with middle cerebral artery occlusion (MCAO). In an in vitro model, the level of miR-152-3p decreased significantly in bEnd.3 cells after oxygen-glucose deprivation/reperfusion insult. Overexpression of miR-152-3p in bEnd.3 cells protected against OGD/R-induced microvascular hyperpermeability. The protective effect of miR-152-3p was associated with reduced endothelial apoptosis through negative modulation of the MAP3K2/JNK/c-Jun pathway.