期刊
CELL
卷 161, 期 5, 页码 1046-1057出版社
CELL PRESS
DOI: 10.1016/j.cell.2015.04.042
关键词
-
资金
- VENI fellowships [91696087, 700.10.402]
- VIDI fellowships [91710330, 91711366]
- Dutch Organization of Scientific Research (NWO) [823.02.017, 175.010.2007.00, 834.11.002]
- Dutch Cancer Society (KWF) [HUBR 2009-4621, VU 2009-3775]
- European Research Council [260627, 336540]
- Association for International Cancer Research (AICR) [13-0297]
- European Research Council (ERC) [260627] Funding Source: European Research Council (ERC)
Most cancer cells release heterogeneous populations of extracellular vesicles (EVs) containing proteins, lipids, and nucleic acids. In vitro experiments showed that EV uptake can lead to transfer of functional mRNA and altered cellular behavior. However, similar in vivo experiments remain challenging because cells that take up EVs cannot be discriminated from non-EV-receiving cells. Here, we used the Cre-LoxP system to directly identify tumor cells that take up EVs in vivo. We show that EVs released by malignant tumor cells are taken up by less malignant tumor cells located within the same and within distant tumors and that these EVs carry mRNAs involved in migration and metastasis. By intravital imaging, we show that the less malignant tumor cells that take up EVs display enhanced migratory behavior and metastatic capacity. We postulate that tumor cells locally and systemically share molecules carried by EVs in vivo and that this affects cellular behavior.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据