The DNA origami technique allows precise positioning of nanoscale objects, facilitating the development of functional nanomechanical devices for investigating DNA-protein interactions. In this study, a nanoscale device was fabricated using the DNA origami technique to study DNA bending proteins. The bending of DNA caused by the TATA-box binding protein (TBP) was observed using a transmission electron microscope, and the roles of transcription factor II A (TF(II)A) and transcription factor II B (TF(II)B) were investigated.
The DNA origami technique offers precise positioning of nanoscale objects with high accuracy. This has facilitated the development of DNA origami-based functional nanomechanical devices that enable the investigation of DNA-protein interactions at the single particle level. Herein, we used the DNA origami technique to fabricate a nanoscale device for studying DNA bending proteins. For a proof of concept, we used TATA-box binding protein (TBP) to evaluate our approach. Upon binding to the TATA box, TBP causes a bend to DNA of similar to 90 degrees. Our device translates this bending into an angular change that is readily observable with a conventional transmission electron microscope (TEM). Furthermore, we investigated the roles of transcription factor II A (TF(II)A) and transcription factor II B (TF(II)B). Our results indicate that TF(II)A introduces additional bending, whereas TF(II)B does not significantly alter the TBP-DNA structure. Our approach can be readily adopted to a wide range of DNA-bending proteins and will aid the development of DNA-origami-based devices tailored for the investigation of DNA-protein interactions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据