期刊
NANO LETTERS
卷 22, 期 23, 页码 9732-9740出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.2c04084
关键词
second near-infrared window; ratiometric fluorescence; nanosensor; FRET; biosensing
类别
资金
- National Natural Science Foundation of China (NNSFC) [51961145403, 21725502, 31771195, 81790640, 21701027, 21875043, 82021002]
- Key Basic Research Program and Intergovernmental International Cooperation Project of Science and Technology Commission of Shanghai Municipality [20490710600, 20YF1402200, 20JC1411700, 19490713100, 20S31903700]
Researchers have developed a fluorescent dyad in the NIR-II window, which shows improved spectral fidelity and serum tolerance for monitoring oxidative stress and evaluating therapeutic efficiency in traumatic brain injury.
Ratiometric fluorescence nanosensors provide quantitative biological information. However, spectral shift and distortion of ratiometric nanosensors in biological media often compromise sensing accuracy, limiting in vivo applications. Here, we develop a fluorescent dyad (aBOP-IR1110) in the second near-infrared (NIR-II) window by covalently linking an asym-metric aza-BODIPY with a ONOO--responsive meso-thiocyanine. The dyad encapsulated in the PEGylated nanomicelle largely improves spectral fidelity in serum culture by >9.4 times compared to that of its noncovalent counterpart. The increased molecular weights (>1480 Da) and hydrophobicity (LogP of 7.87-12.36) lock dyads inside the micelles, which act as the shield against the external environment. ONOO--altered intramolecular Fo''rster resonance energy transfer (FRET) generates linear ratiometric response with better serum tolerance, enabling us to monitor the dynamics of oxidative stress in traumatic brain injury and evaluate therapeutic efficiency. The results show high correlation with in vitro triphenyltetrazolium chloride staining, suggesting the potential of NIR-II dyad-doped nanosensor for in vivo high-fidelity sensing applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据