期刊
NANO LETTERS
卷 22, 期 24, 页码 9805-9814出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.2c02437
关键词
optical trapping; molecular concentration; membrane penetration; drug delivery; cytosolic delivery
类别
资金
- JSPS KAKENHI [JP16H02612, JP17H00856, JP21H04964, JP15H03010, JP20K15196]
- JST FOREST Program [JPMJFR201O]
- Leading University
- JSTMirai Program [JP20K15196, JPMJMI18GA]
In this study, we demonstrate the application of light-induced assembly for drug delivery system which enhances the accumulation and penetration of biofunctional molecules in cell membranes through superradiance-mediated photothermal convection.
The light-induced force and convection can be enhanced by the collective effect of electrons (superradiance and red shift) in high-density metallic nanoparticles, leading to macroscopic assembly of target molecules. We here demonstrate application of the light-induced assembly for drug delivery system with enhancement of cell membrane accumulation and penetration of biofunctional molecules including cell-penetrating peptides (CPPs) with superradiancemediated photothermal convection. For induction of photothermal assembly around targeted living cells in cell culture medium, infrared continuous-wave laser light was focused onto high-density gold-particle-bound glass bottom dishes exhibiting plasmonic superradiance or thin gold-film-coated glass bottom dishes. In this system, the biofunctional molecules can be concentrated around the targeted living cells and internalized into them only by 100 s laser irradiation. Using this simple approach, we successfully achieved enhanced cytosolic release of the CPPs and apoptosis induction using a pro-apoptotic domain with a very low peptide concentration (nM level) by light-induced condensation.
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