期刊
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
卷 885, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.mrgentox.2022.503577
关键词
Bisguaiacols; Lignin-derivable; Genotoxicity; Ames test; DNA damage; Comet assay
In this study, the genotoxicity of six lignin-derivable bisguaiacols was investigated using a multi-tiered method. The results showed that most of the lignin-derivable bisguaiacols were non-mutagenic and non-genotoxic at low concentrations, indicating their potential as safer alternatives to BPA and bisphenol F.
Bisguaiacols, lignin-derivable bisphenols, are considered promising and possibly safer alternatives to bisphenol A (BPA), but comprehensive toxicity investigations are needed to ensure safety. Most toxicity studies of BPA and its analogues have focused on potential estrogenic activity, and only limited toxicological data are available on other toxicity aspects, such as genotoxicity at low exposure levels. In this study, the genotoxicity of six lignin-derivable bisguaiacols with varying regioisomer contents and degrees of methoxy substitution was investi-gated using a multi-tiered method, consisting of in silico simulations, in vitro Ames tests, and in vivo comet tests. The toxicity estimation software tool, an application that predicts toxicity of chemicals using quantitative structure-activity relationships, calculated that the majority of the lignin-derivable bisguaiacols were non-mutagenic. These results were supported by Ames tests using five tester strains (TA98, TA100, TA102, TA1535, and TA1537) at concentrations ranging from 0.5 pmol/plate to 5 nmol/plate. The potential genotoxicity of bisguaiacols was further evaluated using in vivo comet testing in fetal chicken livers, and in addition to the standard alkaline comet assay, the formamidopyrimidine DNA glycosylase enzyme-modified comet assay was employed to investigate oxidative DNA damage in the liver samples. The oxidative stress analyses indicated that the majority of lignin-derivable analogues showed no signs of mutagenicity (mutagenic index < 1.5) or geno-toxicity, in comparison to BPA and bisphenol F, likely due to the methoxy groups on the lignin-derivable aro-matics. These findings reinforce the potential of lignin-derivable bisphenols as safer alternatives to BPA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据