期刊
MOLECULES
卷 28, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/molecules28031013
关键词
kaempferol; myricetin; lipoic acid; erythrocytes; Langmuir method; BAM methods; microelectrophoresis
The influence of kaempferol, myricetin, and lipoic acid on the properties of natural erythrocytes and biological membrane models was investigated. The results showed that these modifications caused changes in the surface charge density and the isoelectric point value.
The influence of kaempferol (K), myricetin (M) and lipoic acid (LA) on the properties of natural erythrocytes, isolated from animal blood and biological membrane models (monolayers and liposomes) made of phosphatidylcholine (PC), cholesterol (CHOL), and sphingomyelin (SM), CHOL in a ratio of 10:9, was investigated. The Langmuir method, Brewster angle microscopy (BAM) and microelectrophoresis were used. The presented results showed that modification of liposomes with kaempferol, myricetin and lipoic acid caused changes in the surface charge density and the isoelectric point value. Comparing the tested systems, several conclusions were made. (1) The isoelectric point for the DPPC:Chol:M (similar to 2.2) had lower pH values compared to lipoic acid (pH similar to 2.5) and kaempferol (pH similar to 2.6). (2) The isoelectric point for the SM-Chol with myricetin (similar to 3.0) had lower pH values compared to kaempferol (pH similar to 3.4) and lipoic acid (pH similar to 4.7). (3) The surface charge density values for the DPPC:Chol:M system in the range of pH 2-9 showed values from 0.2 to -2.5 x 10(-2) C m(-2). Meanwhile, for the DPPC:Chol:K and DPPC:Chol:LA systems, these values were higher at pH similar to 2 (0.7 x 10(-2) C m(-2) and 0.8 x 10(-2) C m(-2)) and lower at pH similar to 9 (-2.1 x 10(-2) C m(-2) and -1.8 x 10(-2) C m(-2)), respectively. (4) The surface charge density values for the SM:Chol:M system in the range of pH 2-9 showed values from 0.5 to -2.3 x 10(-2) C m(-2). Meanwhile, for the DPPC:Chol:K and DPPC:Chol:LA systems, these values were higher at pH similar to 2 (0.8 x 10(-2) C m(-2)), and lower at pH similar to 9 (-1.0 x 10(-2) C m(-2) and -1.8 x 10(-2) C m(-2)), respectively. (5) The surface charge density values for the erythrocytes with myricetin in the range of pH 2-9 showed values from 1.0 to -1.8 x 10(-2) C m(-2). Meanwhile, for the erythrocytes:K and erythrocytes:LA systems, these values, at pH similar to 2, were 1.3 x 10(-2) C m(-2) and 0.8 x 10(-2) C m(-2) and, at pH similar to 9, -1.7 x 10(-2) C m(-2) and -1.0 x 10(-2) C m(-2), respectively.
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