期刊
JOURNAL OF PHYCOLOGY
卷 53, 期 2, 页码 245-260出版社
WILEY
DOI: 10.1111/jpy.12470
关键词
autocatalytic cell death; caspase; Coomassie staining particles; exopolymer particle; hydrogen peroxide; phytoplankton; programmed cell death; SYTOX Green; transparent exopolymer particles
资金
- National Science Foundation (USA) from the Division of Ocean Sciences [OCE 0726369]
- National Science Foundation (China) [31500411]
- Key Laboratory for Ecological Environment in Coastal Areas, State Oceanic Administration [201504]
- Key Laboratory of Integrated Marine Monitoring and Applied Technologies [MATHAB201501]
- Director Grant for Oceanic Technology of South China Sea Branch, State Administration Oceanic [1501]
Exopolymer particles are found throughout the ocean and play a significant biogeochemical role in carbon cycling. Transparent exopolymer particles (TEP) are composed of acid polysaccharides, and Coomassie staining particles (CSP) are proteins. TEPs have been extensively studied in the ocean, while CSP have been largely overlooked. The objective of this research was to determine the role of stress and cell permeability in the formation of TEP and CSP. The diatom Thalassiosira weissflogii and cyanobacterium Synechococcus elongatus were grown in batch cultures and exposed to hydrogen peroxide (0, 10, and 100 mu M) as an environmental stressor. There was no correlation between TEP and CSP concentrations, indicating that they are different populations of particles rather than different chemical components of the same particles. CSP concentrations were not affected by hydrogen peroxide concentration and did not correlate with indicators of stress and cell death. In contrast, TEP concentrations in both taxa were correlated with a decrease in the effective quantum yield of photosystem II, increased activity of caspase-like enzymes, and an increase in the proportion of the population with permeable cell membranes, indicating that TEP production was associated with the process of cell death. These data show that different environmental factors and physiological processes affected the production of TEP and CSP by phytoplankton. TEP and CSP are separate populations of exopolymer particles with potentially different biogeochemical roles in the ocean.
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