期刊
MOLECULES
卷 27, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/molecules27227733
关键词
allergens; bee pollen; MALDI-TOF; mass spectrometry; proteomics
资金
- Polish National Science Centre [2016/23/D/NZ7/03949]
Bee pollen is a valuable medicinal and nutritional product, but its consumption may cause adverse effects due to its unknown composition. This study identified bioactive proteins, including potential allergens, in bee pollen collected in Poland. Standardizing bee pollen is crucial due to its content of both beneficial and harmful compounds, and awareness of allergens can prevent health risks associated with consuming these popular superfoods.
Bee pollen, because of its high content of nutrients, is a very valuable medicinal and nutritional product. However, since its composition is not completely studied, the consumption of this product may cause adverse effects, including allergic reactions. Therefore, this study aimed to discover and characterize the bioactive proteins of bee pollen collected in Poland, focusing mainly on the allergens. For this purpose, the purified and concentrated pollen aqueous solutions were analyzed using the nanoLC-MALDI-TOF/TOF MS analytical platform. As a result of the experiments, 197 unique proteins derived from green plants (Viridiplantae) and 10 unique proteins derived from bees (Apis spp.) were identified. Among them, potential plant allergens were discovered. Moreover, proteins belonging to the group of hypothetical proteins, whose expression had not been confirmed experimentally before, were detected. Because of the content of bioactive compounds-both beneficial and harmful-there is a critical need to develop guidelines for standardizing bee pollen, especially intended for consumption or therapeutic purposes. This is of particular importance because awareness of the allergen content of bee pollen and other bee products can prevent health- or life-threatening incidents following the ingestion of these increasingly popular superfoods.
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