期刊
MOLECULES
卷 27, 期 23, 页码 -出版社
MDPI
DOI: 10.3390/molecules27238496
关键词
bacterial laccase; N-carboxymethyl chitosan; gallic acid; grafting; strawberry preservation
资金
- National Natural Science Foundation of China
- Postgraduate Research & Practice Innovation Program of Jiangsu Province
- [31871743]
- [KYCX22_0723]
Bio-enzymatic grafting of phenolic acid to chitosan derivative is an efficient and environmentally friendly synthesis technology. The grafting of gallic acid to N-carboxymethyl chitosan was successfully achieved using recombinant bacterial laccase. The GA-g-CMCS coating showed positive effects on maintaining the freshness and prolonging the shelf life of strawberries.
Bio-enzymatic grafting phenolic acid to chitosan derivative is an efficient and environmentally friendly molecular synthesis technology. In the present study, N-carboxymethyl chitosan (CMCS) was grafted with gallic acid (GA) using recombinant bacterial laccase from Streptomyces coelicolor as a catalyst. GA and CMCS were successfully grafted as determined by measuring amino acid content, Fourier transform infrared (FTIR) spectroscopy and ultraviolet-visible (UV-Vis) spectroscopy. Then, the effect of GA-g-CMCS coating on the freshness of strawberries at 20 +/- 2 degrees C was explored. The physiological and biochemical quality indicators of strawberries during storage were monitored. The 1.5% GA-g-CMCS coating helped to protect the antioxidant properties and nutrients of strawberries and extend the shelf life. Specifically, it reduced the weight loss of strawberries during preservation (originally 12.7%) to 8.4%, maintained titratable acidity content (TA) residuals above 60% and reduced decay rate from 36.7% to 8.9%. As a bioactive compound, GA-g-CMCS has the potential to become an emerging food packing method. These results provide a theoretical basis and reference method for the subsequent synthesis and application of CMCS derivatives.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据