期刊
MOLECULES
卷 27, 期 23, 页码 -出版社
MDPI
DOI: 10.3390/molecules27238228
关键词
Glycine Willd; flavonols; laser microscopy; HPLC-MS; MS; tandem mass spectrometry; polyphenols
资金
- N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)
- [0662-2019-0006]
This research presents a detailed comparative analysis of bioactive substances in different soybean varieties using advanced microscopy and mass spectrometry techniques. The results reveal that phenolic compounds in soybeans are mainly located in the seed coat and the outer layer of the cotyledon.
In this research, we present a detailed comparative analysis of the bioactive substances of soybean varieties k-11538 (Russia), k-11559 (Russia), k-569 (China), k-5367 (China), k-5373 (China), k-5586 (Sweden), and Primorskaya-86 (Russia) using an LSM 800 confocal laser microscope and an amaZon ion trap SL mass spectrometer. Laser microscopy made it possible to clarify in detail the spatial arrangement of the polyphenolic content of soybeans. Our results revealed that the phenolics of soybean are spatially located mainly in the seed coat and the outer layer of the cotyledon. High-performance liquid chromatography (HPLC) was used in combination with an amaZon SL BRUKER DALTONIKS ion trap (tandem mass spectrometry) to identify target analytes in soybean extracts. The results of initial studies revealed the presence of 63 compounds, and 45 of the target analytes were identified as polyphenolic compounds.
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