4.7 Review

Cellulose synthesis in land plants

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Summary: ECMs play a crucial role in supporting cell integrity and function, as well as in regulating intercellular communication, and are of interest for biofuel production and antimicrobial treatments. Techniques such as ssNMR and MAS-DNP are powerful for understanding the structure and dynamics of carbohydrates and biomolecules in ECMs. Recent high-resolution investigations have revealed structural principles in ECMs across various organisms, and advancements in ssNMR technology offer promising opportunities for further exploration of biochemical topics.

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FLA11 and FLA12 glycoproteins fine-tune stem secondary wall properties in response to mechanical stresses

Yingxuan Ma et al.

Summary: This study suggests that FLA11 and FLA12 may act as cell surface sensors in plant xylem cells, allowing them to sense mechanical stimuli and regulate secondary cell wall (SCW) development. Overexpression of FLA11 promotes earlier SCW development, while overexpression of FLA12 increases cellulose content. These proteins play a role in modulating SCW responses to mechanical stress.

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The carbohydrate-active enzyme database: functions and literature

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Super-resolution imaging illuminates new dynamic behaviors of cellulose synthase

Sydney G. Duncombe et al.

Summary: Confocal imaging has limitations in exploring the dynamic behaviors of CESA particles, but Structured Illumination Microscopy (SIM) provides a two-fold improvement in resolution and reveals new insights into the dense arrangement of cellulose. SIM data show coordinated control of CESA catalytic activity, variability in CESA track patterns, and highlight SIM as a powerful tool for advancing CESA imaging beyond conventional light microscopy.

PLANT CELL (2022)

Review Biochemistry & Molecular Biology

Cell biology of primary cell wall synthesis in plants

Ying Gu et al.

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Plant cell walls as mechanical signaling hubs for morphogenesis

Kristoffer Jonsson et al.

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Cryo-electron tomography of the onion cell wall shows bimodally oriented cellulose fibers and reticulated homogalacturonan networks

William J. Nicolas et al.

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CURRENT BIOLOGY (2022)

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Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components

Dana A. Dahhan et al.

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PLANT CELL (2022)

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Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis

Wei Wang et al.

Summary: The role of SUS in cellulose biosynthesis in Arabidopsis has been refuted, as alternative UDP-glucose biosynthesis pathways were found to be employed.

PLANT JOURNAL (2022)

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Daniel J. Cosgrove

Summary: This article discusses the evolution of primary cell wall structure and the mechanism of surface enlargement, highlighting the role of cellulose, xyloglucan, and pectins. By using molecular dynamics methods, a quantitative model is developed to understand the mechanical behavior of the cell wall, revealing the dominant role of cellulose-cellulose interactions in forming a strong yet extensible network.

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Molecular studies of cellulose synthase supercomplex from cotton fiber reveal its unique biochemical properties

Xingpeng Wen et al.

Summary: The study revealed the existence of a unique cellulose synthase supercomplex in cotton fibers, involved in the synthesis of secondary cell walls. Knocking out key genes led to the disappearance of this supercomplex, resulting in failure to thicken the SCW in cotton fibers. Additionally, cotton fiber CSS exhibited higher enzyme activity and a distinct microfibril structure.

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Secondary cell wall patterning-connecting the dots, pits and helices

Huizhen Xu et al.

Summary: The organization and behavior of microtubules play a crucial role in the formation of plant cell walls and the synthesis of cellulose. Small GTPases control the microtubule patterns. This article gives an overview of the process of wall formation in water-transporting xylem vessels and discusses the challenges in studying this field.

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Evidence for Plant-Conserved Region Mediated Trimeric CESAs in Plant Cellulose Synthase Complexes

Juan Du et al.

Summary: Higher plants use membrane-bound cellulose synthase complexes to synthesize cellulose, with homomeric trimers possibly playing an important role in cell wall synthesis, and key residues for future studies have been identified.

BIOMACROMOLECULES (2022)

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Peripheral membrane proteins modulate stress tolerance by safeguarding cellulose synthases

Christopher Kesten et al.

Summary: This study identifies TTL proteins as new members of the cellulose synthase complex (CSC), which play a crucial role in adjusting cellulose synthesis and maintaining plant growth under stressful conditions.

SCIENCE ADVANCES (2022)

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Regulation of cellulose synthesis via exocytosis and endocytosis

Yu Zhu et al.

Summary: Cellulose is a crucial component of plant cell walls, and its synthesis is regulated by the activity of cellulose synthase enzymes at the plasma membrane, as well as the processes of exocytosis and endocytosis. Recent studies have provided insights into the molecular mechanisms of CESA regulation through mutant analysis and live-cell imaging.

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Delphine Menard et al.

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PLANT CELL (2022)

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Glycosylphosphatidylinositol anchor lipid remodeling directs proteins to the plasma membrane and governs cell wall mechanics

Zuopeng Xu et al.

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Characteristics of Cellulose Nanofibrils from Transgenic Trees with Reduced Expression of Cellulose Synthase Interacting 1

Simon Jonasson et al.

Summary: Cellulose nanofibrils can be influenced in quality by genetic engineering of trees.

NANOMATERIALS (2022)

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An atlas of Arabidopsis protein S-acylation reveals its widespread role in plant cell organization and function

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Cellulose synthesis complexes are homo-oligomeric and hetero-oligomeric in Physcomitrium patens

Xingxing Li et al.

Summary: Mosses contain homo-oligomeric and hetero-oligomeric cellulose synthesis complexes, with different isoforms of cellulose synthase playing distinct roles in primary and secondary cell wall deposition. PpCESA5 in moss Physcomitrium patens is unique in its ability to sustain gametophore morphogenesis without the presence of other isoforms, indicating a specialized function. Additionally, normal protonemal tip growth in the moss requires specific combinations of CESA isoforms from both class A and class B.

PLANT PHYSIOLOGY (2022)

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ENB1 encodes a cellulose synthase 5 that directs synthesis of cell wall ingrowths in maize basal endosperm transfer cells

Qun Wang et al.

Summary: Development of the endosperm differs significantly between monocots and dicots, with persistent endosperm in monocots and transient endosperm in dicots. The mechanisms controlling these differences are not well understood. In this study, a maize mutant, enb1, was characterized, and it was found that the endosperm degradation was drastically increased during kernel development. The ENB1 gene was identified as a key regulator involved in cellulose synthesis and nutrient uptake in the endosperm cells. Additionally, ENB1 expression was found to be induced by sucrose, and overexpression of ENB1 enhanced sucrose transport and contributed to the formation of a persistent endosperm in the kernel.

PLANT CELL (2022)

Review Chemistry, Multidisciplinary

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Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen

Pia Guadalupe Dominguez et al.

Summary: The study reveals the significant impact of SUS on C metabolism and biomass formation in entire trees, confirming its regulatory role in C flow in different tissues and highlighting its importance in wood development.

NEW PHYTOLOGIST (2021)

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Posttranscriptional regulation of cellulose synthase genes by small RNAs derived from cellulose synthase antisense transcripts

Daniel B. Nething et al.

Summary: The study highlights the potential regulatory role of sRNAs derived from CESA antisense transcripts in the control of cell wall biosynthesis, especially during the transition from primary to secondary cell wall biosynthesis. The data suggest that CESA antisense transcripts may function through an RNA-induced silencing mechanism, influencing the expression of cell wall gene networks.

PLANT DIRECT (2021)

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Thomas H. Wilson et al.

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PLANT METHODS (2021)

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Xiangnan Zhang et al.

Summary: Cellulose is a abundant organic polymer in nature synthesized by cellulose synthases in the plasma membrane of higher plants. This study reports the structure of the homotrimeric CesA7 from Gossypium hirsutum, providing insight into how eukaryotic cellulose synthase assembles and offering a mechanistic basis for improving cotton fiber quality in the future.

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Klaas Yperman et al.

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Natalie Hoffmann et al.

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Heather E. McFarlane et al.

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Shiori Terada et al.

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PLANT MOLECULAR BIOLOGY (2021)

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Yao Zhang et al.

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Prashanth Ramachandran et al.

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Tamara Vellosillo et al.

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Rene Schneider et al.

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