4.7 Article

Genomic and phenotypic signatures of bacteriophage coevolution with the phytopathogen Pseudomonas syringae

期刊

MOLECULAR ECOLOGY
卷 -, 期 -, 页码 -

出版社

WILEY
DOI: 10.1111/mec.16850

关键词

bacteriophage evolution; evolutionary history; experimental evolution; genotype-phenotype relationship; plant pathogen

向作者/读者索取更多资源

The rate and trajectory of evolution in an obligate parasite is highly influenced by its host(s). This study investigates the impact of evolutionary history of bacterial hosts on the genotypic and phenotypic evolution of bacteriophages. By comparing coevolved phages and phages passaged on an ancestral strain, it is revealed that coevolution leads to more mutations in phage genomes, particularly in genes encoding phage tail-associated proteins. Coevolved phages exhibit more efficient reduction of population growth and have more predictable effects on infectivity range.
The rate and trajectory of evolution in an obligate parasite is critically dependent on those of its host(s). Adaptation to a genetically homogeneous host population should theoretically result in specialization, while adaptation to an evolving host population (i.e., coevolution) can result in various outcomes including diversification, range expansion, and/or local adaptation. For viruses of bacteria (bacteriophages, or phages), our understanding of how evolutionary history of the bacterial host(s) impacts viral genotypic and phenotypic evolution is currently limited. In this study, we used whole genome sequencing and two different metrics of phage impacts to compare the genotypes and phenotypes of lytic phages that had either coevolved with or were repeatedly passaged on an unchanging (ancestral) strain of the phytopathogen Pseudomonas syringae. Genomes of coevolved phages had more mutations than those of phages passaged on a constant host, and most mutations were in genes encoding phage tail-associated proteins. Phages from both passaging treatments shared some phenotypic outcomes, including range expansion and divergence across replicate populations, but coevolved phages were more efficient at reducing population growth (particularly of sympatric coevolved hosts). Genotypic similarity correlated with infectivity profile similarity in coevolved phages, but not in phages passaged on the ancestral host. Overall, while adaptation to either host type (coevolving or ancestral) led to divergence in phage tail proteins and infectivity patterns, coevolution led to more rapid molecular changes that increased bacterial killing efficiency and had more predictable effects on infectivity range. Together, these results underscore the important role of hosts in driving viral evolution and in shaping the genotype-phenotype relationship.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据