期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 162, 期 -, 页码 213-222出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2016.06.046
关键词
Silver nanoparticles; UVA; 8-hydroxy-2 '-deoxyguanosine; DNA double-strand breaks; gamma-H2AX; Glutathione
资金
- Ministry of Health, Labour and Welfare, Japan
- Japan Society for the Promotion of Science KAKENHI [15H02828]
- Grants-in-Aid for Scientific Research [15H02828] Funding Source: KAKEN
Owing to the wide application of silver nanoparticles (AgNPs), the assessment of health risks associated with their use is of great importance. In this study, we revealed that the potential genotoxicity of AgNPs was enhanced by ultraviolet A (UVA) exposure. Three cultured cell lines were treated with AgNPs, followed by exposure to UVA. AgNPs induced phosphorylation of histone H2AX (gamma-H2AX) following the formation of DNA double-strand breaks (DSBs), which was synergistically enhanced by UVA exposure. Enhanced gamma-H2AX was observed only in cell lines that positively took up AgNPs, and microsized Ag particles, which were difficult to incorporate into cells, showed no gamma-H2AX. Incorporation of AgNPs was not increased by UVA exposure. AgNO3 treatment followed by UVA exposure also induced a marked increase in gamma-H2AX, indicating that the enhanced gamma-H2AX was attributed to Ag ions released from AgNPs. Ag ions reacted with the -SH group of antioxidant molecules, such as glutathione, and induced intracellular oxidative conditions. 8-Hydroxy-2'-deoxyguanosine was formed in the cells treated with AgNPs, which was augmented by UVA irradiation, suggesting that intracellular oxidation caused oxidative DNA damage, leading to the enhanced formation of DSBs and gamma-H2AX. Ag has been considered a safe metal; however, our results provide important insights into the influence of sunlight on the genotoxic potency of AgNPs. (C) 2016 Elsevier B.V. All rights reserved.
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