4.7 Review

Recent advances in nanotechnology-enhanced biosensors for α-fetoprotein detection

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Hierarchical nickel hydroxide nanosheets grown on hollow nitrogen doped carbon nanoboxes as a high-performance surface substrate for alpha-fetoprotein cancer biomarkers electrochemical aptasensing

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Summary: In recent years, the combination of aptamers with new nanomaterials has significantly improved the performance of biosensors, particularly in the design of hollow carbon-based materials. This study successfully developed hierarchical porous nickel hydroxide nanosheets on hollow nitrogen-doped carbon nanoboxes, demonstrating sensitive detection of alpha-fetoprotein content.

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Summary: In this study, black phosphorus nanosheets (BPNSs) were synthesized and loaded with Fe3+ to improve their electrochemical properties. Immunosensors based on Fe3+/BPNSs were constructed to detect AFP, showing good linear relationship and low detection limit.

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Summary: A novel electrochemical immunosensor utilizing polydopamine-coated Fe3O4 nanoparticles, polyaniline, and gold nanoparticles has been developed for ultra-sensitive detection of alpha-fetoprotein, offering high selectivity, repeatability, and stability.

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Detection of Alpha-Fetoprotein Using Aptamer-Based Sensors

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Summary: This review discusses two types of aptamer-based AFP detection methods, optical and electrochemical biosensors. Optical biosensors utilize techniques such as Raman spectroscopy, resonance light-scattering, fluorescence, and chemiluminescence, while electrochemical biosensors use cyclic voltammetry, electrochemical impedance spectroscopy, and giant magnetic impedance. Future research will focus on developing AFP detection methods that are highly sensitive, stable, cost-effective, and easy to operate.

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Summary: Early detection of cancer biomarkers is crucial for effective diagnosis and treatment of cancer. A sandwich immunoassay based on SERS technology was developed for the ultrasensitive detection of alpha-fetoprotein as a biomarker for early-stage hepatocellular carcinoma, showing promising potential for clinical applications in early cancer detection.

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Sunflower-Like Nanostructure with Built-In Hotspots for Alpha-Fetoprotein Detection

Xiaoyu Zhao et al.

Summary: A sunflower-like nanostructure array was created in this study using colloidal lithography, with synaptic nanoparticles contributing to the primary electromagnetic field. This structure, used as a surface-enhanced Raman spectroscopy active substrate, demonstrated high repeatability and sensitivity in rapid detection of ultra-low concentrations of Alpha-fetoprotein. The design of this plasmonic structure with strong electromagnetic coupling shows promise for clinical medicine applications.

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