期刊
MICROCHIMICA ACTA
卷 189, 期 12, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-022-05570-5
关键词
Aptasensor; Fluorescence; FRET; IL-6; Gold nanoparticles; Nitrogen-doped carbon quantum dots
In this study, a label-free and specific FRET-based IL-6 aptasensor was developed. The biosensor showed high sensitivity, excellent selectivity, and could be applied for IL-6 detection in clinical settings.
A label-free and specific FRET-based interleukin-6 (IL-6) aptasensor was developed using a DNA aptamer modified with nitrogen-doped carbon quantum dots (NCDs) and gold nanoparticles (AuNPs) as a donor-quencher pair. The assayed target was capable of disrupting the donor-acceptor assemblies yielding a concentration-related fluorescence recovery of NCDs (lambda(em) = 445 nm and lambda(ex) = 350 nm). By designing two different probes, the interaction of DNA aptamers with IL-6 protein was studied using FRET efficiency. It appeared that the sensing probes showed slightly different sensing profiles. One of the aptasensors showed a linear response of 1.5-5.9 pg/mL for IL-6 with a coefficient of determination of R-2 >= 0.99 and the a detection limit of 0.82 pg/mL (at S/N = 3). The experimental results indicated that the biosensor can be applied to determine IL-6 in human serum (with recovery of 95.7-102.9%). Due to the high sensitivity, excellent selectivity, and simplicity of the procedure, this strategy represents a promising alternative for IL-6 sensing in clinical applications.
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