4.7 Article

A new method for determination of polysaccharides in adsorption of Hg2+

期刊

MICROCHEMICAL JOURNAL
卷 183, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.microc.2022.107962

关键词

Extracellular polysaccharides; Hg2+; Adsorption; Fluorescence indicator; G-quadruplex; Thioflavin T

资金

  1. National Natural Science Foundation of China [31900038]
  2. Natural Science Foundation of Hunan province, China [2021JJ40377, 2021JJ3029]
  3. Shanxi Province Science Foundation for Youths [201801D221284]
  4. Scientific and Technological Innovation Projects of Colleges and Universities in Shanxi Province [2019L0675]
  5. Independent Exploration and Innovation project for postgraduate of Hunan [CX20200297]
  6. Research Fund of The State Key Laboratory of Coal Resources and safe Mining [SKLCRSM22KF020]

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A new label-free fluorescent sensor has been developed for detecting Hg2+ adsorbed by EPS. This sensor allows for the detection of Hg2+ in aqueous solution using fluorescence spectroscopy, and has been used to successfully analyze the adsorption properties of different EPS on Hg2+. It provides an effective detection technique for heavy metal pollution treatment using EPS.
Bacterial extracellular polysaccharides (EPS) has a good heavy metal ion adsorption function and can be used in sewage treatment of heavy metal pollution. In this work, we develop a new, label-free, turn-on fluorescent sensor for detecting EPS adsorbed Hg2+. This approach is based on the conformational switch from G-quadruplex complex induced by fluorogenic dye Thioflavin T (ThT) to Hg(2+)mediated T-Hg-T double-stranded DNA formation. By this method, Hg2+ in aqueous solution can be detected by fluorescence spectroscopy in the range of 5-1200 nM. The entire reaction and analysis of Hg2+ was performed in 10 min under optimal experimental conditions (1.4 mu M ThT, 0.35 mu M TBA, 1.2 mu M Hg2+ and 10 min incubation time at 25 degrees C, pH7.5). Then, we have utilized this facile approach to detect the adsorption properties of different EPS on Hg2+ with fluorescence spectrometry. These EPS were produced by the fermentation of Pseudoalteromonas sp. BAY, Bacillus sp. RS1, Mesonia sp. Hua40 and Bacillus sp3. SL7 respectively. The Hg(2+ )adsorption capacity of EPS is between 80.1 mg/g to 169.6 mg/g. Among them, the extracellular polysaccharides of Mesonia sp. Hua40 have the strongest Hg(2+ )adsorption capacity, about 169.6 mg/g. This method provides an effective detection technique to analyze Hg2+ adsorption capacity of EPS.

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